TMT10plex™ Isobaric Label Reagents and Kits
TMT10plex™ Isobaric Label Reagents and Kits
TMT10plex™ Isobaric Label Reagents and Kits
TMT10plex™ Isobaric Label Reagents and Kits
TMT10plex™ Isobaric Label Reagents and Kits
TMT10plex™ Isobaric Label Reagents and Kits
Thermo Scientific™

TMT10plex™ Isobaric Label Reagents and Kits

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter.
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Catalog NumberQuantityProduct Type
904061 x 5 mg (per tag), 60 reactionsIsobaric Label Reagent Set
901101 x 0.8 mg (per tag), 10 reactionsIsobaric Label Reagent Set
901113 x 0.8 mg (per tag), 30 reactionsIsobaric Label Reagent Set
903098 x 0.2 mgIsobaric Label Reagent Set
901133 x 0.8 mgIsobaric Mass Tag Labeling Kit
Catalog number 90406
Price (EUR)
5.222,65
Online Exclusive
5.940,00
Save 717,35 (12%)
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Quantity:
1 x 5 mg (per tag), 60 reactions
Product Type:
Isobaric Label Reagent Set
Recurring order eligible. Learn more »
Price (EUR)
5.222,65
Online Exclusive
5.940,00
Save 717,35 (12%)
Each
Add to cart
Ask our AI about this Product
TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. Up to ten different peptide samples prepared from cells or tissues can be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131 Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

The Tandem Mass Tag (TMT) Isobaric Label reagents and kits are specifically designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT10plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments.

Advantages of the TMT10plex label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or improving statistics on biological replicates.

Features of TMT10plex Isobaric Label reagent sets and mass tagging kits
Powerful—multiplexed MS analysis increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues, or biological fluids
Consistent—identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex, and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
Compatible—optimized for use with high resolution Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3.

Applications:
• Protein identification and quantitation from multiple samples of cells, tissue, or biological fluids
• Protein expression profiling of normal vs. disease states or control vs. treated
• Quantitative analysis of proteins for which no antibodies are available
• Identification and quantitation of membrane and post-translationally modified proteins
• Identification and quantification of hundreds to thousands of proteins in a single experiment

TMT Reagents are provided as standalone sets, a 96-well format, or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability. When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex reagents provide integrated total solutions for quantitative protein expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

General References:

  1. Thompson, A., et al.(2003). Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem.75(8), 1895-1904.
  2. Dayon, L., et al.(2008). Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags. Anal. Chem. 80(8), 2921 -2931.
Specifications
Final Product TypeProtein PEPTIDE
For Use With (Equipment)Mass Spectrometer
Label or DyeTandem Mass Tag™ (TMT) Labels
Quantity1 x 5 mg (per tag), 60 reactions
Sufficient For60 Reactions
Workflow StepProtein Labeling
Detection MethodMass Spectrometry
Product LineTMT10plex™
Product TypeIsobaric Label Reagent Set
Starting MaterialCell Lysate
Unit SizeEach
Contents & Storage
Sufficient for: Six 10-plex (6 x 10-way) experiments
• TMT10-126 Label Reagent, 1 x 5 mg
• TMT10-127N Label Reagent, 1 x 5 mg
• TMT10-127C Label Reagent, 1 x 5 mg
• TMT10-128N Label Reagent, 1 x 5 mg
• TMT10-128C Label Reagent, 1 x 5 mg
• TMT10-129N Label Reagent, 1 x 5 mg
• TMT10-129C Label Reagent, 1 x 5 mg
• TMT10-130N Label Reagent, 1 x 5 mg
• TMT10-130C Label Reagent, 1 x 5 mg
• TMT10-131 Label Reagent, 1 x 5 mg

Store at –20°C.

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Lot #Certificate TypeDateCatalog Number(s)
3213020Certificate of AnalysisJun 14, 202590406
3213020ACertificate of AnalysisJun 14, 202590406
AA394198Certificate of AnalysisApr 15, 202590309
3213302PRODUCT DATA SHEETJun 02, 202590110
3213213PRODUCT DATA SHEETJun 02, 202590110
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Limited Use Label Licenses (LULL)

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The purchase of this product conveys to the buyer the non-exclusive, non-transferable right to use the product and components of the product for internal research purposes of the buyer only, whether the buyer is an academic or for-profit entity. This product is not available for use in diagnostic procedures.

The buyer must obtain a license for use of the product in performing commercial services or for any other commercial purposes. For information on purchasing a license, contact proteomics.licensing@thermofisher.com, Pierce Biotechnology, Inc., 3747 North Meridian Road, Rockford, IL 61101.

If the buyer is not willing to accept the limitations of this limited use statement, Pierce Biotechnology, Inc. is willing to accept return of the unused product with a full refund. By opening and using this product, the buyer agrees to be bound by the conditions of this Limited Use Label License.

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Citations & References (8)

Citations & References
Abstract
Autophagy Regulates Plant Tolerance to Submergence by Modulating Photosynthesis.
Authors:Yang M,Wei J,Xu Y,Zheng S,Yu B,Ming Y,Jin H,Xie L,Qi H,Xiao S,Huang W,Chen L
Journal:Plant, cell & environment
PubMed ID:39575938
The increase in global climate variability has increased the frequency and severity of floods, profoundly affecting agricultural production and food security worldwide. Autophagy is an intracellular catabolic pathway that is dispensable for plant responses to submergence. However, the physiological role of autophagy in plant response to submergence remains unclear. In ... More
Downregulation of STAT1 improved learning and memory impairments in aging mice.
Authors:Li X,Xu Y,Li T,Xiong B,Yang X,Feng Y
Journal:Neuroscience letters
PubMed ID:39933634
Cognitive impairment is a typical hallmark of aging in mice and humans. Here, we reported that downregulation of STAT1 improved learning and memory impairments in aging mice by enhancing the expression of synaptic protein and inhibiting the expression of inflammatory factors. Proteomic analysis revealed 139 differentially expressed proteins (DEPs) in ... More
Tandem Mass Tags for Comparative and Discovery Proteomics.
Authors:Pagel O,Kollipara L,Sickmann A
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:33950487
Relative or comparative proteomics provides valuable insights about the altered protein abundances across different biological samples in a single (labeled) or series (label-free) of LC-MS measurement(s). Chemical labeling of peptides using isobaric mass tags for identification and quantification of different proteomes simultaneously has become a routine in the so-called discovery ... More
Authors:
Journal:
PubMed ID:39178843
A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans.
Authors:Shivarathri R,Chauhan M,Mazumdar R,Trinh PC,Reiter W,Hartl M,Kuchler K,Chauhan N
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:36008655
Candida albicans is a normal component of the human microflora that colonizes mucosal/epithelial surfaces and the gastrointestinal tract as a commensal organism. However, in an immunocompromised host, it can cause life-threatening infections of high mortality and morbidity. Virulence as well as antifungal drug resistance of C. albicans is often regulated ... More
8 total citations

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