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TMTpro™ 16plex Label Reagent Set
TMTpro™ 16plex Label Reagent Set
Thermo Scientific™

TMTpro™ 16plex Label Reagent Set

The Thermo Scientific TMTpro 16plex Label Reagent Set enables multiplexing of up to 16 samples for multiplexed protein identification and quantitation using tandem mass spectrometry (MS/MS).
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Catalog NumberQuantity
A445201 x 5 mg (per tag)
A445211 x 0.5 mg (per tag)
A445226 x 0.5 mg (per tag)
A583346 x 50 μg, 96 reactions
Catalog number A44520
Price (USD)
7,794.50
Special offer
Online exclusive
Ends: 15-Aug-2025
9,170.00
Save 1,375.50 (15%)
Each
In stock
Add to cart
Quantity:
1 x 5 mg (per tag)
Recurring order eligible. Learn more »
Price (USD)
7,794.50
Special offer
Online exclusive
Ends: 15-Aug-2025
9,170.00
Save 1,375.50 (15%)
Each
Add to cart
Ask our AI about this Product

TMTpro label reagents are the next generation of tandem mass tags, designed to increase multiplexing without compromising protein identification and quantitation. The structures of the TMTpro and TMT tags are similar in being isobaric and amine reactive, but the TMTpro tag has a longer spacer region and isobutyl proline mass reporter. After fragmentation, each TMTpro tag generates a unique reporter mass (i.e., TMTpro 126-134Da) in the low-mass region of the high-resolution MS/MS spectrum used for multiplexed relative quantitation of protein expression.

The advantages of TMTpro tags include increased multiplexed relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. With these capabilities, the TMTpro 16plex label reagents are suited for applications requiring higher multiplexing and fewer missing values, such as inhibitor dose response experiments, time course experiments, thermal shift assays, or single cell analysis. When combined with the industry leading, high-resolution Orbitrap instruments and software, TMTpro reagents provide integrated total solutions for quantitative protein expression analysis.

Features of TMTpro 16plex Label Reagent Sets include:
Multiplex—concurrent MS analysis of up to 16 samples derived from cells, tissues, or biological fluids
Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
Efficient—amine-reactive, NHS ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
Compatible—optimized for use with high resolution Thermo Scientific Orbitrap MS/MS platforms, such as the Q Exactive and Orbitrap Fusion Tribrid instrument series, including the Orbitrap Eclipse Tribrid and Ascend and Orbitrap Exploris 480 mass spectrometers with data analysis fully supported by Proteome Discoverer 2.3

For high resolution analysis of TMTpro-labeled peptides, the recommended LC column for the Nanospray Flex source is the Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For the EASY-Spray source, the recommended LC column is the EASY-Spray C18 LC Column (Cat. No. ES902 or ES003).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePeptide
For Use With (Equipment)Mass Spectrometer
Label or DyeTandem Mass Tag™ (TMT) Labels
Quantity1 x 5 mg (per tag)
Shipping ConditionDry Ice
Workflow StepProtein Labeling
Detection MethodMass Spectrometry
Product LineTMT™
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Unit SizeEach
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Frequently asked questions (FAQs)

What is the shelf life for the TMTpro 16plex Label Reagent Set?

The TMTpro 16plex Label Reagent Set is guaranteed for one year from the date of receipt in original, unopened packaging. This information can also be found in the Certificate of Analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the TMT and TMTpro reagents be mixed?

No, the TMT and TMTpro reagents cannot be mixed because the reporter ions are in the same region.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can TMTpro label reagents dissolved in anhydrous acetonitrile be dried down and stored for later use?

Yes, unused TMTpro label reagent that has been dissolved in anhydrous acetonitrile can be aliquoted into tubes, dried under vacuum at 25-30 degrees C, purged with nitrogen, and stored in a sealed bag with desiccant at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What size formats is the TMTpro 16plex Label Reagent Set available in?

The TMTpro 16plex Label Reagent Set can be purchased in three different size formats:
1 x 0.5 mg (per tag), Cat No. A44521; sufficient for one 16plex isobaric experiment
6 x 0.5 mg (per tag), Cat No. A44522; sufficient for six 16plex isobaric experiments
1 x 5 mg (per tag), Cat No. A44520; sufficient for ten 16pex isobaric experiments

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

If I already have TMTpro 16-plex tags, what do I need to order to get a total of 18-plex TMTpro tags?

Depending upon the number of vials and amount of each tag in the vial for the TMTpro 16-plex tags you already have, you will have to purchase the appropriate TMTpro-134C and TMTpro-135N Label Reagents.
- If you already have TMTpro 16plex Label Reagent Set, 1 x 5 mg (Cat. No. A44520), you will have to purchase TMTpro-134C and TMTpro-135N Label Reagents, 1 x 5 mg (Cat. No. A52046).
- If you already have TMTpro 16plex Label Reagent Set, 6 x 0.5 mg (Cat. No. A44522), you will have to purchase TMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg (Cat. No. A52048).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3214338PRODUCT DATA SHEETJun 26, 2025A44520
3214336PRODUCT DATA SHEETJun 06, 2025A44520
3213811Certificate of AnalysisJun 14, 2025A44522
3231354Certificate of AnalysisJun 12, 2025A58334
3231353Certificate of AnalysisJun 04, 2025A58334
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Safety Data Sheets

Citations & References (12)

Citations & References
Abstract
Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice.
Authors:Gaun A,Lewis Hardell KN,Olsson N,O'Brien JJ,Gollapudi S,Smith M,McAlister G,Huguet R,Keyser R,Buffenstein R,McAllister FE
Journal:Journal of proteome research
PubMed ID:33499602
Performing large-scale plasma proteome profiling is challenging due to limitations imposed by lengthy preparation and instrument time. We present a fully automated multiplexed proteome profiling platform (AutoMP3) using the Hamilton Vantage liquid handling robot capable of preparing hundreds to thousands of samples. To maximize protein depth in single-shot runs, we ... More
Discovering the Radiation Biomarkers in the Plasma of Total-Body Irradiated Leukemia Patients.
Authors:Gabriela R,Vera V,Pavel R,Helena R,Igor S,Marie D,Marketa M,Alena MF,Ales T
Journal:Radiation research
PubMed ID:38315067
The increased risk of acute large-scale radiological exposure for the world's population underlines the need for optimal radiation biomarkers. Ionizing radiation triggers a complex response by the genome, proteome, and metabolome, all of which have been reported as suitable indicators of radiation-induced damage in vivo. This study analyzed peripheral blood samples ... More
Improved Proteomics-Based Drug Mechanism-of-Action Studies Using 16-Plex Isobaric Mass Tags.
Authors:Zinn N,Werner T,Doce C,Mathieson T,Boecker C,Sweetman G,Fufezan C,Bantscheff M
Journal:Journal of proteome research
PubMed ID:33621079
Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures changes in protein thermal stability in response to drug treatment and thus informs on direct targets and ... More
Establishing Quality Control Procedures for Large-Scale Plasma Proteomics Analyses.
Authors:Patterson KL,Arul AB,Choi MJ,Oliver NC,Whitaker MD,Bodrick AC,Libby JB,Hansen S,Dumitrescu L,Gifford KA,Jefferson AL,Hohman TJ,Robinson RAS
Journal:Journal of the American Society for Mass Spectrometry
PubMed ID:37163770
Proteomics research has been transformed due to high-throughput liquid chromatography (LC-MS/MS) tandem mass spectrometry instruments combined with highly sophisticated automated sample preparation and multiplexing workflows. However, scaling proteomics experiments to large sample cohorts (hundreds to thousands) requires thoughtful quality control (QC) protocols. Robust QC protocols can help with reproducibility, quantitative ... More
Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry.
Authors:Dey KK,Sun H,Wang Z,Niu M,Wang H,Jiao Y,Sun X,Li Y,Peng J
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:34905163
Mass spectrometry (MS) has become a mainstream platform for comprehensive profiling of proteome, especially with the improvement of multiplexed tandem mass tag labeling coupled with two-dimensional liquid chromatography and tandem mass spectrometry (TMT-LC/LC-MS/MS). Recently, we have established a robust method for direct profiling of undepleted cerebrospinal fluid (CSF) proteome with ... More
12 total citations

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