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PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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| Catalog Number | No. of Reactions |
|---|---|
| K0171 | 200 Reactions |
| K0172 | 1000 Reactions |
Catalog number K0171
Price (EUR)
138,00
Each
-
No. of Reactions:
200 Reactions
Price (EUR)
138,00
Each
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions200 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypePCR Master Mix
Quantity200 reactions
Reaction FormatSuperMix or Master Mix
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 2X PCR Master Mix, 4 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.
• Nuclease-free water, 4 x 1.25 mL
Store at -20°C.
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Lot #Certificate TypeDateCatalog Number(s)
3249854Certificate of AnalysisJun 02, 2025K0172
3245572Certificate of AnalysisMay 26, 2025K0171
3245388Certificate of AnalysisMay 23, 2025K0171
3219578Certificate of AnalysisApr 16, 2025K0172
3206115Certificate of AnalysisApr 07, 2025K0171
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Safety Data Sheets
Product Information
Frequently asked questions (FAQs)
Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.