PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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Catalog Number
Quantity
10572014
100 rxns
Catalog number 10572014
Price (USD)
154.65
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163.00
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100 rxns
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Price (USD)
154.65
Online Exclusive
163.00
Save 8.35 (5%)
Each
Add to cart
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PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg2+, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5 kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1.1X
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartNo
No. of Reactions100 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypePCR SuperMix
Quantity100 rxns
Reaction FormatSuperMix or Master Mix
Shipping ConditionWet or Dry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 4 vials of PCR SuperMix, 1.125 mL each
Store at -20°C.
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Citations & References
Abstract
Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358.
Authors: Saitoh Hisato; Pizzi Maryann Delli; Wang Jian;
Journal:J Biol Chem
PubMed ID:11709548
Ubc9, a conjugation enzyme for the ubiquitin-related modifier SUMO, is present predominantly in the nucleus and at the nuclear pore complex. The functional significance of its subcellular compartmentalization, however, remains to be elucidated. Here, we define a Pro-Glu-Asp-Ser-Thr-rich element containing 129 amino acid residues, designated IR1+2, on the human nucleoporin ... More
Lithocholic acid decreases expression of bile salt export pump through farnesoid X receptor antagonist activity.
Authors: Yu Jinghua; Lo Jane-L; Huang Li; Zhao Annie; Metzger Edward; Adams Alan; Meinke Peter T; Wright Samuel D; Cui Jisong;
Journal:J Biol Chem
PubMed ID:12052824
Bile salt export pump (BSEP) is a major bile acid transporter in the liver. Mutations in BSEP result in progressive intrahepatic cholestasis, a severe liver disease that impairs bile flow and causes irreversible liver damage. BSEP is a target for inhibition and down-regulation by drugs and abnormal bile salt metabolites, ... More