Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity | Separation Range | Stain Type | Gel Percentage |
---|---|---|---|---|
G720841 | 4 x 8 Gels | 400 to 10,000 bp | SYBR™ Safe | 1% |
G700801 | 8 Gels | 400 to 10,000 bp | Ethidium Bromide | 1% |
G720801 | 8 Gels | 400 to 10,000 bp | SYBR™ Safe | 1% |
G700802 | 8 Gels | 100 to 2000 bp | Ethidium Bromide | 2% |
G720802 | 8 Gels | 100 to 2000 bp | SYBR™ Safe | 2% |
G720842 | 4 x 8 Gels | 100 to 2000 bp | SYBR™ Safe | 2% |
Invitrogen E-Gel 96 high-throughput precast agarose gels with or without SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis. Each E-Gel agarose gel cassette contains all components required for efficient gel separation and analysis—just load your samples and run.
Each gel contains 96 sample lanes and 8 marker lanes, ideal for screening multiple PCR products, plasmid preparations, and restriction digests. The E-Gel 96 loading format is compatible with multi-channel pipettors and the most commonly used 8-, 12-, and 96-pin liquid handling robots. With a 12-minute run time, up to 20,000 samples can be resolved in a single day.
The unique 96-well staggered-well format provides a 1.6-cm run length with the following separation ranges for DNA fragments:
Each cassette is labeled with an unique EAN39 bar code that is recognized by most commercially available robotic bar code readers.
Features of E-Gel 96 gels include:
Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.
Unlike ethidium bromide, the DNA stain most commonly incorporated into agarose gels, SYBR Safe DNA Gel Stain is a non-toxic, non-mutagenic DNA stain that is safer for the user, safer for the environment, and can save institutional overhead costs for disposal.
Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel staining steps. Just load your samples and run.
Running and imaging devices
High-throughput E-Gel agarose gels require the E-Base Electrophoresis Device for gel running.
Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The high-throughput E-Gel 96 and 48 agarose gels with or without SYBR Safe are available in 1% and 2% gel percentages.
SYBR Safe-stained gels can be visualized with a standard UV transilluminator, a laser-based scanner equipped with an excitation source in the UV range or between 470 and 530 nm, or a blue-light transilluminator.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Here are some suggestions:
- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.
Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.
Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:
E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA
Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
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