β-Gal Staining Kit
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Invitrogen™

β-Gal Staining Kit

El kit de tinción β-Gal le permite determinar el porcentaje de células transfectadas que expresan lacZ. El lacZ es unMás información
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Número de catálogoCantidad
K1465011 kit
Número de catálogo K146501
Precio (EUR)
856,00
Each
Disponibilidad estimada 11-Aug-2025
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Cantidad:
1 kit
Recurring order eligible. Learn more »
Precio (EUR)
856,00
Each
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El kit de tinción β-Gal le permite determinar el porcentaje de células transfectadas que expresan lacZ. El lacZ es un gen bacteriano que a menudo se utiliza como constructor de indicadores en experimentos de transfección eucariota. El producto genético del lacZ, la β-galactosidasa, es resistente a la proteólisis en lisados celulares y su actividad se analiza fácilmente. La β-galactosidasa cataliza la hidrólisis de X-gal, produciendo un color azul que puede verse fácilmente bajo el microscopio.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoEnsayo de β-Gal (lacZ)
Tipo de productoKit de tinción Beta-Gal
Cantidad1 kit
SustratoX-Gal
ObjetivoBeta-galactosidasa
Método de detecciónColorimétrico
Unit SizeEach
Contenido y almacenamiento
El kit de tinción β-Gal contiene suficientes reactivos para teñir cincuenta placas de 60 mm. El kit incluye PBS (solución salina tamponada con fosfato) (10), X-gal, soluciones de tinción A, B y C, 10 soluciones fijadoras y 10 µg de vector de control pcDNA™ 3.1/His/lacZ. Guarde los 10 PBS a temperatura ambiente. Todos los demás componentes deben almacenarse a -20°C. Se garantiza la estabilidad de todos los reactivos durante 6 meses si se almacenan correctamente.
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Preguntas frecuentes

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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Citations & References (3)

Citations & References
Abstract
Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses.
Authors:Cashion LM, Bare LA, Harvey S, Trinh Q, Zhu Y, Devlin JJ
Journal:Biotechniques
PubMed ID:10337486
'Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
Authors:Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
Journal:J Immunol
PubMed ID:15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Phosphorylation of serine 256 is required for cAMP-dependent regulatory exocytosis of the aquaporin-2 water channel.
Authors:Fushimi K, Sasaki S, Marumo F
Journal:J Biol Chem
PubMed ID:9169447
The aquaporin-2 (AQP2) vasopressin water channel is translocated to the apical membrane upon vasopressin stimulation. Phosphorylation of serine 256 of AQP2 by cAMP-dependent protein kinase has been shown, but its relation to vasopressin-regulated translocation has not been elucidated. To address this question, wild type (WT) AQP2 and a mutant with ... More
3 total citations

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