X-Gal Solution, ready-to-use
X-Gal Solution, ready-to-use
Thermo Scientific™

X-Gal Solution, ready-to-use

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, formingRead more
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Catalog NumberQuantity
R094110 mL
Catalog number R0941
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152.65
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Price (USD)
152.65
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177.00
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Each
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Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Applications

• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies
• Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells
• Detection of beta-galactosidase activity in immunological and histochemical procedures

Related Products
X-Gal
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeX-Gal Solution
Quantity10 mL
Substrate PropertiesChemical Substrate
Substrate TypeBeta-Gal Substrate, X-Gal
Detection MethodColorimetric
Unit SizeEach
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Lot #Certificate TypeDateCatalog Number(s)
3272622Certificate of AnalysisJul 02, 2025R0941
3272607Certificate of AnalysisJul 02, 2025R0941
3240872Certificate of AnalysisMay 19, 2025R0941
3227521Certificate of AnalysisApr 29, 2025R0941
3185846Certificate of AnalysisMar 07, 2025R0941
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