Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Certificates
SDS
Citations & References (12)
Invitrogen™
Dynabeads™ M-280 Sheep Anti-Mouse IgG
Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently boundRead more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| 11202D | 10 mL |
| 11201D | 2 mL |
Catalog number 11202D
Price (HKD)
13,440.00
Each
Quantity:
10 mL
Price (HKD)
13,440.00
Each
Dynabeads M-280 Sheep anti-Mouse IgG beads are 2.8-μm monosized, monodispersed, superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. These beads are intended for use as a solid support for simple and efficient binding of mouse IgGs and their target proteins. The uniform, monodisperse, and non-porous Dynabeads magnetic beads make them an ideal choice for applications such as binding immunoglobulins, protein purification, sandwich immunoassays, immunoprecipitation (IP), Co-immunoprecipitation (Co-IP), and isolation of cells and microorganisms. The polyclonal sheep antibody binds mouse IgG1, IgG2a, and IgG2b, and, with a lower reactivity, mouse IgG3 and IgM. Human cross-reactivity is minimal.
Benefits of Dynabeads M-280 Sheep anti-Mouse IgG beads include:
- Speed—isolate protein in less than 40 minutes
- Selectivity—binds most mouse IgG subclasses, very low non-specific binding
- Flexibility—no columns, centrifugations, or time-consuming pre-clearing required
- Automation ready—automation protocols available with the KingFisher Sample Purification instruments
Great signal to noise ratio
Dynabeads M-280 Sheep anti-Mouse IgG beads covalently couple the secondary sheep antibodies to a hydrophobic surface, which means the non-specific hydrophobic interactions are reduced to a minimum. Since this interaction is the main driver of unwanted protein binding, Dynabeads magnetic beads help ensure a great signal-to-noise ratio. This Dynabeads magnetic bead platform binds any mouse IgG antibody in a solution and can therefore become a powerful tool in affinity purification.
Dynabeads immunoprecipitation protocol is fast and easy
Add the target specific primary mouse IgG antibody directly to the sample (indirect technique) or first pre-coat the target antibody onto the Dynabeads M-280 Sheep Anti-Mouse IgG beads (direct technique). Regardless of which technique is used, when the Dynabeads M-280 Sheep anti-Mouse IgG beads are mixed with the sample, they bind to the target. The manual IP protocol is simple and can be performed in less than 40 minutes with 3 easy bind-incubate-elute steps. The procedure does not require any pre-clearing due to the low non-specific binding and uses very little antibody while still obtaining a high IP yield, thus saving both time and cost per IP.
The antibody-coated beads can be used in a variety of IP applications such as Co-IP, chromatin IP (ChIP/ChIP sequencing), RNA IP (RIP, RIP sequencing), small-scale IgG purification, and protein purification. The isolated pure target protein can be used in downstream western blot, mass spectrometry analysis, sequencing, etc.
Automation-ready for use with KingFisher purification systems
The 2.8-μm magnetic beads are ideal for high-throughput enrichment and can be automated using any of our KingFisher sample purification systems.
Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Sheep anti-Mouse IgG beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.
Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Certifications/ComplianceISO9001 and ISO13485
ColorBrown
Concentration10 mg/mL
DescriptionPolyclonal sheep anti-mouse IgG covalently bound to Dynabeads
Diameter (Metric)2.8 μm
For Use With (Application)Protein Purification
For Use With (Equipment)KingFisher™ Sample Purification Systems, DynaMag™ Magnets
FormatBeads in suspension
High-throughput CompatibilityHigh-throughput Compatible
Ligand TypeAntibody
MaterialPolystyrene
Quantity10 mL
ReactivityBinds mouse IgG1, IgG2a and IgG2b. Low reactivity towards mouse IgG3 and IgM.
Regulatory StatusFor Research Use Only
Shelf Life36 months from date of manufacture
Shipping ConditionAmbient temperature
SpeciesSheep
TargetProteins
UniformityMonosized 2.8 μm (CV <5%)
TypeAntibody Coated Bead
Unit SizeEach
Contents & Storage
Dynabeads M-280 Sheep Anti-Mouse IgG is supplied in PBS, pH 7.4 w/0.1% BSA, 0.02% NaN3 Store at 2°C to 8°C.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Figures
Manual and automated immunoprecipitation of CD9
Non-specific binding with manual and automated immunoprecipitation
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3248618Certificate of AnalysisJun 23, 202511202D
3196796Certificate of AnalysisJun 06, 202511201D
3248623Certificate of AnalysisMay 29, 202511202D
3248612Certificate of AnalysisMay 29, 202511202D
3190892Certificate of AnalysisMay 19, 202511202D
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSScientific Resources
Product Information
Protocols
Frequently asked questions (FAQs)
The background might be caused by nonspecific binding to the BSA on the bead surface. Alternatively, high background might be caused by nonspecific binding to streptavidin. Increasing either the pH or the salt concentration might help reduce the binding. Dynabeads M-270 Streptavidin magnetic beads might be a better alternative; these beads are not coated with BSA and are hydrophilic, as they are based upon carboxylic acid chemistry.
Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:
- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.
Try these suggestions:
- Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.
Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.
For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.
Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.
Yes, Dynabeads magnetic beads can be used to isolate single-stranded DNA. Streptavidin Dynabeads magnetic beads can be used to target biotinylated DNA fragments, followed by denaturation of the double-stranded DNA and removal of the non-biotinylated strand. The streptavidin-coupled Dynabeads magnetic beads will not inhibit any enzymatic activity. This enables further handling and manipulation of the bead-bound DNA directly on the solid phase. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/preparing-single-stranded-dna-templates.html) for more information in regards to single-stranded DNA capture.
Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.
Magnetic susceptibility is a measure of how quickly the beads will migrate to the magnet. This will depend on the iron content and the character of the iron oxide. The magnetic susceptibility given for the Dynabeads magnetic beads is the mass susceptibility, given either as cgs units/g or m^3/kg (the latter being an SI unit). For ferri- and ferromagnetic substances, the magnetic mass susceptibility is dependent upon the magnetic field strength (H), as the magnetization of such substances is not a linear function of H but approaches a saturation value with increasing field. For that reason, the magnetic mass susceptibility of the Dynabeads magnetic beads is determined by a standardized procedure under fixed conditions. The magnetic mass susceptibility given in our catalog is thus the SI unit. Conversion from Gaussian (cgs, emu) units into SI units for magnetic mass susceptibility is achieved by multiplying the Gaussian factor (emu/g or cgs/g) by 4 pi x 10^-3. The resulting unit is also called the rationalized magnetic mass susceptibility, which should be distinguished from the (SI) dimensionless magnetic susceptibility unit. In general, magnetic mass susceptibility is a measure of the force (Fz) influencing an object positioned in a nonhomogenous magnetic field. The magnetic mass susceptibility of the Dynabeads magnetic beads is measured by weighing a sample, and then subjecting the sample to a magnetic field of known strength. The weight (F1) is then measured, and compared to the weight of the sample when the magnetic field is turned off (F0). The susceptibility is then calculated as K x 10^-3 = [(F1-F0) x m x 0.335 x 10^6], where K is the mass susceptibility of the sample of mass m. The susceptibility is then converted to SI units.
Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.
Citations & References (12)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Development and mechanism of ?-secretase modulators for Alzheimer's disease.
Journal:Biochemistry
PubMed ID:23614767
'?-Secretase is an aspartyl intramembranal protease composed of presenilin, Nicastrin, Aph1, and Pen2 with 19 transmembrane domains. ?-Secretase cleaves the amyloid precursor proteins (APP) to release Aß peptides that likely play a causative role in the pathogenesis of Alzheimer's disease (AD). In addition, ?-secretase cleaves Notch and other type I
Characterization of the postsynaptic protein neurogranin in paired cerebrospinal fluid and plasma samples from Alzheimer's disease patients and healthy controls.
Journal:
PubMed ID:26136856
'Synaptic dysfunction and degeneration are central events in Alzheimer''s disease (AD) pathophysiology that are thought to occur early in disease progression. Synaptic pathology may be studied by examining protein biomarkers specific for different synaptic elements. We recently showed that the dendritic protein neurogranin (Ng), including the endogenous Ng peptide 48
Brain amyloid-beta fragment signatures in pathological ageing and Alzheimer's disease by hybrid immunoprecipitation mass spectrometry.
Journal:
PubMed ID:25591542
'Senile plaques in Alzheimer''s disease (AD) are composed of amyloid-ß (Aß), especially N-truncated forms including Aß4-42. These are thought to be neurotoxic. However, individuals may live for decades with biomarker evidence of cerebral ß-amyloidosis (positive amyloid PET imaging and/or low cerebrospinal fluid levels of the 42 amino acid form of
Pyroglutamate amyloid ß (Aß) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease.
Journal:J Biol Chem
PubMed ID:22267726
'Pyroglutamate-modified Aß peptides at amino acid position three (Aß(pE3-42)) are gaining considerable attention as potential key players in the pathogenesis of Alzheimer disease (AD). Aß(pE3-42) is abundant in AD brain and has a high aggregation propensity, stability and cellular toxicity. The aim of the present work was to study the
APP metabolism regulates tau proteostasis in human cerebral cortex neurons.
Journal:
PubMed ID:25921538
'Accumulation of Aß peptide fragments of the APP protein and neurofibrillary tangles of the microtubule-associated protein tau are the cellular hallmarks of Alzheimer''s disease (AD). To investigate the relationship between APP metabolism and tau protein levels and phosphorylation, we studied human-stem-cell-derived forebrain neurons with genetic forms of AD, all of
12 total citations