Pierce™ Western Blot Signal Enhancer
Pierce™ Western Blot Signal Enhancer
Thermo Scientific™

Pierce™ Western Blot Signal Enhancer

Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhanceRead more
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Catalog NumberQuantity
21050Promo Image500 mL
Catalog number 21050
Price (HKD)
1,832.00
Each
Add to cart
Quantity:
500 mL
Recurring order eligible. Learn more »
Price (HKD)
1,832.00
Each
Add to cart
Ask our AI about this Product
Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

Features of Western Blot Signal Enhancer:

Increases protein detection—most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
Improves antibody binding—the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
Works for nearly any protein—signal enhancement has been demonstrated with targets such as IL-6, p53, NFκB, BRCA1 and EGF
Effective with any substrate—enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
Compatible with any membrane—enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
Fast, 15-minute protocol—optimized for a combination of simplicity, speed and signal enhancement for most proteins
Ready-to-use—no formulating or diluting necessary, and the reagents are stable for storage at room temperature

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, Chemiluminescence
For Use With (Application)Western Blot
FormLiquid
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™
Product TypeWestern Blot Enhancer
Quantity500 mL
Unit SizeEach
Contents & Storage
Upon receipt store kit at room temperature.
Have questions about this product? Ask our AI assisted search.
When should I use the Pierce Western Blot Signal Enhancer?
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Can I use the Pierce Western Blot Signal Enhancer on a stripped blot?
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
ZI394573ACertificate of AnalysisOct 15, 202421050
ZI394573Certificate of AnalysisOct 15, 202421050
ZD386692Certificate of AnalysisApr 23, 202421050
YK381497Certificate of AnalysisDec 07, 202321050
YF374888Certificate of AnalysisJun 22, 202321050
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Safety Data Sheets

Molecular Probes® Handbook

Frequently asked questions (FAQs)

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Enhancer does not bind to the membrane or proteins so it should not interfere with an antibody in binding the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After stripping the blot, blocking reagent remains on the membrane so you will get inconsistent results. Therefore, we recommend using the Enhancer only before blocking and the first probing of the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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