NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO
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NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO
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引用資料與參考文獻 (22)
Invitrogen™

NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO

The NeuroTrace green fluorescent Nissl stain, available only from Molecular Probes, binds to the Nissl substance, which is present exclusively深入閱讀
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產品號碼Quantity
N214801 mL
產品號碼 N21480
價格 (HKD)
2,346.00
Each
新增至購物車
Quantity:
1 mL
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價格 (HKD)
2,346.00
Each
新增至購物車
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The NeuroTrace green fluorescent Nissl stain, available only from Molecular Probes, binds to the Nissl substance, which is present exclusively in the somata of neuronal cells. The NeuroTrace Nissl stain exhibits bright, green fluorescence that is visible with filters appropriate for fluorescein and is many times more sensitive than cresyl violet stain. NeuroTrace green fluorescent Nissl stain is an improvement on the classic FluoroNissl Green dye.
For Research Use Only. Not for use in diagnostic procedures.
規格
ColorGreen
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product TypeNissl Stain
Quantity1 mL
Shipping ConditionRoom Temperature
Sub Cellular LocalizationNissl Bodies
Excitation/Emission500/525 nm
Product LineNEUROTRACE™
Unit SizeEach
內容物與存放
Store in freezer (-5 to -30°C) and protect from light.
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常見問答集 (常見問題)

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

圖表

Pyramidal cells of a mouse hippocampus and dentate gyrus. NeuroTrace® green fluorescent Nissl stain.
Pyramidal cells of a mouse hippocampus and dentate gyrus. NeuroTrace® green fluorescent Nissl stain.

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批號Certificate TypeDateCatalog Number(s)
3101974Certificate of AnalysisDec 17, 2024N21480
2725301Certificate of AnalysisNov 03, 2023N21480
2585746Certificate of AnalysisJan 30, 2023N21480
2456934Certificate of AnalysisMar 28, 2022N21480
2291550Certificate of AnalysisMay 20, 2021N21480
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Product Information

Molecular Probes® 手冊

Probes for the Nucleus—Section 12.5
Nucleic Acid Stains—Section 8.1
Polar Tracers—Section 14.3

引用資料與參考文獻 (22)

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引用資料與參考文獻
Abstract
Beyond the olfactory bulb: an odotopic map in the forebrain.
Authors:Nikonov AA, Finger TE, Caprio J
Journal:Proc Natl Acad Sci U S A
PubMed ID:16339016
'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More
JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease.
Authors:Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14704277
'Parkinson''s disease (PD) is a neurodegenerative disorder characterized by loss of dopamine-containing neurons, but the molecular pathways underlying its pathogenesis remain uncertain. Here, we show that by eliminating c-Jun N-terminal kinases (JNKs) we can prevent neurodegeneration and improve motor function in an animal model of PD. First, we found that ... More
Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.
Authors:Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R,
Journal:
PubMed ID:24497575
Spinal muscular atrophy (SMA) is characterized by motor neuron loss, caused by mutations or deletions in the ubiquitously expressed survival motor neuron 1 (SMN1) gene. We recently identified a novel role for Smn protein in glucose metabolism and pancreatic development in both an intermediate SMA mouse model (Smn(2B/-)) and type ... More
Cortical control of adaptation and sensory relay mode in the thalamus.
Authors:Mease RA, Krieger P, Groh A,
Journal:
PubMed ID:24748112
A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and ... More
High-throughput morphometric analysis of individual neurons.
Authors:Wu CC, Reilly JF, Young WG, Morrison JH, Bloom FE
Journal:Cereb Cortex
PubMed ID:15054070
To facilitate high-throughput quantitative analysis of neuronal structure, this study optimized the diOlistic method of whole neuron labeling to examine multiple neurons in fixed brain, and optimized image acquisition parameters to preserve signal for subsequent photoconversion. Fluorescent dye-coated gold particles were successively delivered by helium-powered ejection to 250 microm thick ... More
22 total citations

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