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Invitrogen™
Novex™ Tricine SDS Sample Buffer (2X)
Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. NovexRead more
| Catalog Number | Quantity |
|---|---|
| LC1676 | 20 mL |
Catalog number LC1676
Price (KRW)
42,000
Online offer
Ends: 30-Jun-2025
49,000Save 7,000 (14%)
Each
-
Quantity:
20 mL
Price (KRW)
42,000
Online offer
Ends: 30-Jun-2025
49,000Save 7,000 (14%)
Each
Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. Novex Tricine SDS Sample Buffer is specifically formulated for optimal electrophoresis of small proteins and peptides. The sample buffer is formulated with Coomassie Blue G and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie Blue G gives a sharp dye front with Tricine SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides. This ensures that small peptides do not run off the gels.
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.
Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.
See all buffers and reagents for SDS-PAGE ›
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.
Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.
See all buffers and reagents for SDS-PAGE ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialSample Loading Buffer
Recommended StorageTricine Sample Buffer (2X) containing sodium dodecyl sulfate (SDS) at pH 8.45 with Coomassie Blue G and phenol red.
Store at 2°C to 8°C.
Store at 2°C to 8°C.
Concentration2X
Physical FormLiquid
Product LineNovex
Quantity20 mL
Unit SizeEach
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Frequently asked questions (FAQs)
Adding urea to the sample and running buffers, in conjunction with SDS, may provide improved solubilization of the sample if denaturation by SDS does not prove to be sufficient. This must be tested empirically for the protein of interest.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
You should run the gel until the phenol red tracking dye from the Novex Tricine SDS Sample Buffer (Cat. No. LC1676) reaches the bottom of the gel. Phenol red serves as an indicator of the running front as it is a very small molecule that migrates with the ion front in Tricine gels. The Coomassie from the sample buffer runs a little slower and can be 1-2 cm behind the phenol red.
Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.
Novex Tricine SDS Sample Buffer (2X), does not contain DTT or other reducing agents.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.