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Catalog Number | Quantity |
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R37111 | 1.75 mL kit |
Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.
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It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.
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The recommended storage conditions for CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111) is between 4 and 25 degrees C or room temperature.
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We have tested Caspase 3/7 Green Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) for up to 48 hours and observed no toxicity or stability issues with the reagent. If the samples are fixed at the end of the time course the signal will be retained for days. For live cells, apoptotic cells will round up and float away before the loss of fluorescence signal from CellEvent.
The following reference reports a visible signal for up to 72 hrs:
Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020 May 25;14:1995-2019. doi: 10.2147/DDDT.S242514. PMID: 32546966; PMCID: PMC7260544.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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