CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent
CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent
Invitrogen™

CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation ofRead more
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Catalog NumberQuantity
R371111.75 mL kit
Catalog number R37111
Price (KRW)
373,000
Online offer
Ends: 30-Sep-2025
466,000
Save 93,000 (20%)
Each
In stock
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Quantity:
1.75 mL kit
Recurring order eligible. Learn more »
Price (KRW)
373,000
Online offer
Ends: 30-Sep-2025
466,000
Save 93,000 (20%)
Each
Add to cart
Ask our AI about this Product
CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent™ Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death.

• Simple and fast no-wash protocol helps preserve delicate apoptotic cells
• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.

See other ReadyProbes™ reagents for cell staining
Learn more about other assays for apoptosis

Cell imaging applications
CellEvent™ Caspase-3/7 Green reagent is a four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is non-fluorescent when not bound to DNA. The CellEvent™ Caspase-3/7 Green reagent is intrinsically non-fluorescent, as the DEVD peptide inhibits binding of the dye to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind DNA, generating a bright green fluorescence. The fluorescence emission of the dye when bound to DNA is 530 nm and can be observed using a standard FITC filter set.

Suggestions for use
• In most cases, 2 drops/ml and an incubation time of 30 minutes is sufficient for bright nuclear staining of apoptotic cells; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• If desired, combine CellEvent™ Caspase-3/7 Green ReadyProbes™ reagent with a red or far-red nuclear stain for a total cell count
• CellEvent™ Caspase-3/7 Green dye is excited with a maximum at 502 nm and has an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Compatible CellsMammalian Cells
Excitation/Emission502/530
For Use With (Application)Cell Imaging
For Use With (Equipment)Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
Label TypeOther Label(s) or Dye(s)
Product LineCellEvent™, ReadyProbes™
Product TypeCaspase 3/7 Reagent
Quantity1.75 mL kit
Detection MethodFluorescence
FormatDropper Bottle
Unit SizeEach
Contents & Storage
1 × 1.75 mL dropper bottles

Store at ≤ 25°C.
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Figures

Fluorescence spectra

Fluorescence spectra

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3163206Certificate of AnalysisMay 03, 2025R37111
2971125Certificate of AnalysisOct 16, 2024R37111
2892778Certificate of AnalysisMay 22, 2024R37111
2800310Certificate of AnalysisDec 13, 2023R37111
2714498Certificate of AnalysisAug 31, 2023R37111
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Safety Data Sheets

Frequently asked questions (FAQs)

Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The recommended storage conditions for CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111) is between 4 and 25 degrees C or room temperature.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We have tested Caspase 3/7 Green Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) for up to 48 hours and observed no toxicity or stability issues with the reagent. If the samples are fixed at the end of the time course the signal will be retained for days. For live cells, apoptotic cells will round up and float away before the loss of fluorescence signal from CellEvent.

The following reference reports a visible signal for up to 72 hrs:

Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020 May 25;14:1995-2019. doi: 10.2147/DDDT.S242514. PMID: 32546966; PMCID: PMC7260544.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (29)

Citations & References
Abstract
SHOX triggers the lysosomal pathway of apoptosis via oxidative stress.
Authors:Hristov G, Marttila T, Durand C, Niesler B, Rappold GA, Marchini A,
Journal:
PubMed ID:24186869
The SHOX gene encodes for a transcription factor important for normal bone development. Mutations in the gene are associated with idiopathic short stature and are responsible for the growth failure and skeletal defects found in the majority of patients with Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia. SHOX is expressed ... More
Disruption of mitochondrial quality control genes promotes caspase-resistant cell survival following apoptotic stimuli.
Authors:
Journal:J Biol Chem
PubMed ID:35304098
Design and synthesis of novel 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4-thiadiazol- 2-yl)urea derivatives with potent anti-CML activity throughout PI3K/AKT signaling pathway.
Authors:Li W, Chu J, Fan T, Zhang W, Yao M, Ning Z, Wang M, Sun J, Zhao X, Wen A
Journal:Bioorg Med Chem Lett
PubMed ID:31097376
'In this investigation, a series of 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4- thiadiazol-2-yl)urea receptor tyrosine kinase inhibitors were synthesized by a simple and efficient structure-based design. Structure-activity relationship (SAR) analysis of these compounds based on cellular assays led to the discovery of a number of compounds that showed potent activity against human chronic myeloid leukemia ... More
SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial ?? in neuroblastoma cells.
Authors:Tanpure S, Boyineini J, Gnanamony M, Antony R, Fernández KS, Libes J, Lin J, Pinson D, Joseph PA, Gondi CS
Journal:Oncol Lett
PubMed ID:28599461
'Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27)' ... More
The Development of a Nano-based Approach to Alleviate Cisplatin-Induced Ototoxicity.
Authors:Kayyali MN, Ramsey AJ, Higbee-Dempsey EM, Yan L, O'Malley BW, Tsourkas A, Li D
Journal:J Assoc Res Otolaryngol
PubMed ID:29349595
'Cisplatin-induced hearing loss is experienced by a high percentage of patients with squamous cell carcinoma undergoing cisplatin chemotherapy. A novel nano-construct capable of sequestering extracellular cisplatin was developed to combat this problem. The nano-construct consisted of superparamagnetic iron oxide nanoparticles (SPIONs) entrapped within polymeric micelles, which were formed from a ... More
29 total citations

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