Search Thermo Fisher Scientific
Certificates
SDS
Citations & References (7)
Invitrogen™
CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent
CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. TheRead more
PromotionPromo code:RFLOW25
Get more, spend less on every flow cytometry run
Fuel discovery without breaking your budget. Save up to 33% on flow cytometry essentials—compensation beads, buffers, assays & more. Use code RFLOW25 at checkout. The more you buy, the more you save. Limited time only!Learn More
| Catalog Number | Quantity |
|---|---|
| R37167 | 120 assays |
Catalog number R37167
Price (USD)
544.00
Each
In stock
Quantity:
120 assays
Price (USD)
544.00
Each
CellEvent™ Caspase-3/7 Green Ready Flow™ Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Exceptionally bright green fluorescence upon binding to nucleic acids
• Rapid staining of apoptotic cells without wash steps
CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Exceptionally bright green fluorescence upon binding to nucleic acids
• Rapid staining of apoptotic cells without wash steps
CellEvent Caspase-3/7 Green Ready Flow Reagent is a cell-permeant reagent that detects caspase 3/7 activity using flow cytometry. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
CellEvent Caspase-3/7 Green Ready Flow Reagent consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorescent signal having absorption/emission maxima of ∼502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.
Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.
Specifications
ColorGreen
Excitation/Emission504/523
Label TypeOther Label(s) or Dye(s)
Label or DyeCellEvent™ Caspase 3/7 Green, SYTOX AADvanced Dead Cell Stain
Product LineCellEvent™ Ready Flow
Product TypeCaspase 3/7 Reagent
Quantity120 assays
Shipping ConditionRoom Temperature
Sub Cellular LocalizationNucleus
FormatDropper Bottle
Unit SizeEach
Contents & Storage
4 dropper bottles, store at room temperature
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Frequently asked questions (FAQs)
Figures
Jurkat cells treated with CellEvent Caspase 3/7 Green reagent
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3001520Certificate of AnalysisApr 02, 2025R37167
2980693Certificate of AnalysisSep 18, 2024R37167
2896539Certificate of AnalysisMar 14, 2024R37167
2860800Certificate of AnalysisJan 05, 2024R37167
2765693Certificate of AnalysisDec 13, 2023R37167
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSProduct Information
Citations & References (7)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Alternatives to current flow cytometry data analysis for clinical and research studies.
Journal:Methods
PubMed ID:29305968
'Flow cytometry has well-established methods for data analysis based on traditional data collection techniques. These techniques typically involved manual insertion of tube samples into an instrument that, historically, could only measure 1-3 colors. The field has since evolved to incorporate new technologies for faster and highly automated sample preparation and
Measurement and Characterization of Apoptosis by Flow Cytometry.
Journal:Curr Protoc Cytom
PubMed ID:27367289
'Apoptosis is an important mechanism in cell biology, playing a critical regulatory role in virtually every organ system. It has been particularly well characterized in the immune system, with roles ranging from immature immune cell development and selection to down-regulation of the mature immune response. Apoptosis is also the primary
Computational flow cytometry reveals that cryopreservation induces spermptosis but subpopulations of spermatozoa may experience capacitation-like changes.
Journal:Reproduction
PubMed ID:27965398
'The reduced lifespan of cryopreserved spermatozoa in the mare reproductive tract has been attributed to both capacitative and apoptotic changes. However, there is a lack of studies investigating both phenomena simultaneously. In order to improve our knowledge in this particular point, we studied in raw and frozen-thawed samples apoptotic and
New flow cytometry approaches in equine andrology.
Journal:Theriogenology
PubMed ID:27160445
Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the
Effects of ghrelin on the apoptosis of human neutrophils in vitro.
Journal:Int J Mol Med
PubMed ID:27431014
Acute respiratory distress syndrome (ARDS) is characterized by lung inflammation and the diffuse infiltration of neutrophils into the alveolar space. Neutrophils are abundant, short-lived leukocytes that play a key role in immune defense against microbial infections. These cells die via apoptosis following the activation and uptake of microbes, and will also
7 total citations