DS-02 Matrix Standard Kit, for 3500/3730/SeqStudio™/SeqStudio™ Flex
DS-02 Matrix Standard Kit, for 3500/3730/SeqStudio™/SeqStudio™ Flex
Applied Biosystems™

DS-02 Matrix Standard Kit, for 3500/3730/SeqStudio™/SeqStudio™ Flex

The dye-labeled oligonucleotides included in the Matrix Standard DS-02 set (dR110, dR6G, dTAMRA, dROX, and LIZ) are used to generate자세히 알아보기
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카탈로그 번호수량
43230148 Runs
카탈로그 번호 4323014
제품 가격(KRW)
327,000
Each
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수량:
8 Runs
Recurring order eligible. Learn more »
제품 가격(KRW)
327,000
Each
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The dye-labeled oligonucleotides included in the Matrix Standard DS-02 set (dR110, dR6G, dTAMRA, dROX, and LIZ) are used to generate the 'multicomponent matrix' required for the SNaPshot Multiplex Kit. The Data Collection software utilizes the multicomponent matrix to automatically correct for the spectral overlap in samples labeled with DS-02 dyes. The kit consists of one tube of matrix standard which is sufficient for a minimum of eight array runs (on a 24 capillary) or two array runs (on a 96 capillary).

Matrix standards do not need to be run with every set of sample injections. The standard only needs to be run once in order to generate a matrix file which is then applied to samples run under similar conditions. For more information on the use of matrix standards, refer to the instrument User Manual or Getting Started Guide.

Used with SNaPshot Multiplex Kit on 3500/3500xl, 3730/3730xl, SeqStudio, and SeqStudio Flex series genetic analyzers.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Sequencing
용도(장비)SeqStudio™ Genetic Analyzer, 3730 Series Genetic Analyzers, 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers
라벨 또는 염료LIZ, dRhodamine 6G, dRhodamine ROX, dRhodamine TAMRA
제품 유형DS-02 Matrix Standard Kit
수량8 Runs
배송 조건Wet Ice
형식Kit
Unit SizeEach
구성 및 보관
Contains dye-labeled oligonucleotides used to generate the 'multicomponent matrix' required for the SNaPshot™ Multiplex Kit. Store at 2 - 8°C. Do not freeze.
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Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
3256133Certificate of Analysis2025년 6월 13일4323014
3203275Certificate of Analysis2025년 6월 02일4323014
3201350Certificate of Analysis2025년 4월 01일4323014
3170647Certificate of Analysis2025년 3월 24일4323014
3130825Certificate of Analysis2025년 2월 03일4323014
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Safety Data Sheets | 물질 안전 보건 자료

라벨 라이센스 사용이 제한된 항목

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Notice to Purchaser: This product is optimized for use in the DNA sequencing or fragment analysis methods covered by patents owned and/or controlled by Life Technologies Corporation (“LTC”). LTC does not convey any right or license under these patents, whether expressly, by implication, by estoppels, or otherwise, to the purchaser by the purchase of this product to use the DNA sequencing or fragment analysis methods. Notwithstanding the foregoing, a limited license to use the DNA sequencing or fragment analysis methods covered by such patents can be obtained for certain research and development activities (a) through the purchase of certain LTC reagents when such reagents are used in conjunction with an authorized LTC instrument, or (b) directly from LTC. For information on obtaining additional rights to practice the DNA sequencing or fragment analysis methods, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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자주 묻는 질문(FAQ)

A spectral calibration is an algorithm applied to raw data, which converts it into the component 4 or 5 dye data stored in the sample files. A spectral is created for a specific dye set (combination of dyes), array type (4 or 16 capillaries), and array length (36cm or 50cm). It is used to correct for the natural overlap of the fluorescent dyes.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

DS-02 (Dye Set E5), DS-30 (Dye Set D), DS-31 (Dye Set D), DS-32 (Dye Set F), and DS-33 (Dye Set G5) are all supported on the Applied Biosystems 3130 Series systems. Please refer to the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide (http://tools.thermofisher.com/content/sfs/manuals/cms_041468.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

If the peaks in other colors are directly below the peak of interest, the issue could be that the fluorescent dye being used is not part of the selected dye set, the spectral calibration needs to be performed, or the peaks are offscale. Confirm that the dye set selected on the instrument is compatible with the dye being used, run a new spectral calibration if the correct dye set has been selected and, if the signal intensity is too high, decrease sample concentration during PCR or when preparing samples for electrophoresis.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

The SNaPshot Multiplex Kit is designed to interrogate up to ten single nucleotide polymorphisms (SNPs) at known locations on one to ten DNA templates in a single tube.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Follow these recommendations for designing and evaluating primers:

- Primers included in a single reaction need to differ significantly in lengths in order to avoid overlap between the final SNaPshot products. A difference of 4-6 nucleotides between primer lengths is recommended as a starting point (5-7 nucleotides if running on POP-7 Polymer).
- The length of a primer can be modified by the addition of non-homologous polynucleotides at the 5′ end. Since the recommended annealing temperature for a SNaPshot control primer is 50 degrees C, the melting temperature for the complementary region between any primer and its corresponding template should be at least 50 degrees C.
- Poly (dT), poly (dA), poly (dC), and poly (dGACT) are 5′ non-homologous tails which are predicted to have minimal secondary structures. They have all been used successfully. Generally the signal patterns are not affected by the kinds of tails that are used. The 5′ poly (dT) tails however may interfere with the addition of 3′ ddA.
- The mobility of an oligonucleotide in capillary electrophoresis is determined by its size, nucleotide composition, and dye. Thus the effect of nucleotide composition on mobility can be significant when the primer is short. We strongly recommend that you test primers shorter than 36 nucleotides before being multiplexed to ensure that the final products are spatially resolved when analyzed on the instrument.
- Check primers for possible extendable hairpin structures within each primer and for extendable dimer formation between primers.
- HPLC purification of primers is recommended for oligonucleotides longer than 30 nucleotides. Heterogeneous primer mixtures containing mixed molecular weight oligonucleotides may yield undesired products that will confuse analysis.

For additional suggestions please refer to Appendix A of the SNaPshot Multiplex Kit manual (https://tools.thermofisher.com/content/sfs/manuals/cms_041203.pdf).

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

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