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인용 및 참조 문헌 (1122)
Applied Biosystems™
High-Capacity cDNA Reverse Transcription Kit
High Capacity cDNA Reverse Transcription Kit (구 High Capacity cDNA Archive Kit)는 주입 총 RNA 0.02 ∼2 μg 에서 매우자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 포함 | 반응 수 |
|---|---|---|
| 4368813 | Kit only | 1,000 Reactions |
| 4368814 | Kit only | 200 Reactions |
| 4374966 | Kit with Rnase Inhibitor | 200 Reactions |
| 4374967 | Kit with Rnase Inhibitor | 1,000 Reactions |
카탈로그 번호 4368813
제품 가격(KRW)
1,566,000
Online offer
Ends: 30-Sep-2025
1,739,000할인액 173,000 (10%)
Each
재고 있음
포함:
Kit only
반응 수:
1,000 Reactions
제품 가격(KRW)
1,566,000
Online offer
Ends: 30-Sep-2025
1,739,000할인액 173,000 (10%)
Each
High Capacity cDNA Reverse Transcription Kit (구 High Capacity cDNA Archive Kit)는 주입 총 RNA 0.02 ∼2 μg 에서 매우 우수한 single-stranded cDNA를 추출합니다. cDNA 저장소 생성에 이상적임. High Capacity cDNA Reverse Transcription Kit는 최대 2 μg의 input total RNA을 단일 20 μl 반응에 전환시킵니다. 100 μl까지 반응을 확장시켜 단일 반응에서 10 μg cDNA 까지 생성할 수 있습니다. 생성된 cDNA는 real-time PCR, standard PCR, microarray 등 다양한 어플리케이션에 이상적입니다.
• 역동 범위 – 역동 범위 10배
• 민감성 – 0.02 ∼2 μg input total RNA에서 cDNA를 생성합니다.
• 활용성 – 저농도 GC, AU-rich 표적 등 다양한 RNA template과 저농도 발현 RNA에서 시험되었습니다.
이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
• 역동 범위 – 역동 범위 10배
• 민감성 – 0.02 ∼2 μg input total RNA에서 cDNA를 생성합니다.
• 활용성 – 저농도 GC, AU-rich 표적 등 다양한 RNA template과 저농도 발현 RNA에서 시험되었습니다.
이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도50 U/μL
최종 제품 유형First-Strand cDNA
형식Kit
포함Kit only
반응 수1,000 Reactions
수량Each
반응 형식Separate components
시약 유형Reverse Transcription
역전사 효소MultiScribe
리보뉴클레아제 H 활성Yes
배송 조건Dry Ice
크기(최종 제품)7 kb
시작 물질RNA
기술Reverse Transcription
용도(애플리케이션)Real Time PCR (qPCR), RT-PCR
반응 속도120 min.
Unit SizeEach
구성 및 보관
• 10X RT Buffer (2 x 1 mL)
• 10X RT Random Primers (2 x 1 mL)
• 25X dNTP Mix (1.0 mL at 100 mM)
• MultiScribe Reverse Transcriptase (1.0 mL at 50 U/μL)
Store at –15 to –25°C.
Have questions about this product? Ask our AI assisted search.
Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?
Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?
Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?
What is the concentration of the random primers in the High-Capacity cDNA Reverse Transcription Kit (Cat. no. 4368813)?
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Certificates | 증명서
Lot 번호 또는 부분 Lot 번호로 검색하십시오.
Lot 번호Certificate TypeDateCatalog Number(s)
3274538Certificate of Analysis2025년 7월 04일4374967
3273365Certificate of Analysis2025년 7월 03일4368814
3271396Certificate of Analysis2025년 7월 01일4368813
3270174Certificate of Analysis2025년 6월 27일4368814
3269125Certificate of Analysis2025년 6월 26일4374966
시험 성적서 관련 5 개의 결과가 검색되었습니다.
Safety Data Sheets | 물질 안전 보건 자료
SDS자주 묻는 질문(FAQ)
You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.
We recommend the following kits:
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
The concentration of the "random primers" in the High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813) is 25 µM. We recommend that you use these primers at a 1X concentration (2.5 µM) in reverse transcription reactions.
Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.
It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).
인용 및 참조 문헌 (1122)
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인용 및 참조 문헌
Abstract
Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.
Journal:The Journal of biological chemistry
PubMed ID:18077444
Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.
Journal:Toxicological sciences : an official journal of the Society of Toxicology
PubMed ID:15673846
4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell
TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.
Journal:J Biol Chem
PubMed ID:21324893
MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of
Somatic cell plasticity and Niemann-Pick type C2 protein: fibroblast activation.
Journal:J Biol Chem
PubMed ID:21084287
A growing body of evidence points toward activated fibroblasts, also known as myofibroblasts, as one of the leading mediators in several major human pathologies including proliferative fibrotic disorders, invasive tumor growth, rheumatoid arthritis, and atherosclerosis. Niemann-Pick Type C2 (NPC2) protein has been recently identified as a product of the second
Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
Journal:J Exp Med
PubMed ID:19652016
The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance of bacteria penetrating the lining of the bowel and predispose to granuloma formation and chronicity. We tested this hypothesis in human subjects by monitoring responses to
1122 total citations