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Gibco™
DR4 Mouse Embryonic Fibroblasts, irradiated
Irradiated Gibco DR4 Mouse Embryonic Fibroblasts (MEFs) are multidrug-resistant feeder cells that support the culture of healthy undifferentiated human and자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| A34966 | 2 x 106 cells |
카탈로그 번호 A34966
제품 가격(KRW)
169,000
Online offer
Ends: 30-Sep-2025
187,000할인액 18,000 (10%)
Each
재고 있음
수량:
2 x 106 cells
제품 가격(KRW)
169,000
Online offer
Ends: 30-Sep-2025
187,000할인액 18,000 (10%)
Each
Irradiated Gibco DR4 Mouse Embryonic Fibroblasts (MEFs) are multidrug-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6204G, these MEF cells are isolated from DR4 x CF1 hybrid mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or CF6-Neo Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with MEFs that are resistant to neomycin/geneticin, puromycin, hygromycin, and 6-thioguanine
Help save time with ready-to-use MEFs
Irradiated DR4 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of DR4 MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.
Perform drug selection with multidrug-resistant cells
DR4 MEFs express transgenes that make them resistant to four different drugs: geneticin (G418), puromycin, hygromycin, and 6-thioguanine. Each lot is tested for tolerance to various concentrations of these drugs and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing drug selection.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with MEFs that are resistant to neomycin/geneticin, puromycin, hygromycin, and 6-thioguanine
Help save time with ready-to-use MEFs
Irradiated DR4 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of DR4 MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.
Perform drug selection with multidrug-resistant cells
DR4 MEFs express transgenes that make them resistant to four different drugs: geneticin (G418), puromycin, hygromycin, and 6-thioguanine. Each lot is tested for tolerance to various concentrations of these drugs and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing drug selection.
For Research Use Only. Not for use in diagnostic procedures.
사양
공여자 소스Pooled
셀 수2 x 106
수량2 x 106 cells
배송 조건Shipped on dry ice
AgeEmbryonic
셀 유형Feeder Cells (Mouse Embryonic Fibroblast)
형태Cryopreserved
종Mouse
Unit SizeEach
구성 및 보관
Cryopreserved mouse embryonic fibroblasts
Store in liquid nitrogen.
Store in liquid nitrogen.
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DR4 Mouse Embryonic Fibroblasts, irradiated
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Certificates | 증명서
Lot 번호 또는 부분 Lot 번호로 검색하십시오.
Lot 번호Certificate TypeDateCatalog Number(s)
2611603Certificate of Analysis2025년 1월 29일A34966
2819726Certificate of Analysis2025년 1월 27일A34966
2935029Certificate of Analysis2025년 1월 26일A34966
2978343Certificate of Analysis2025년 1월 26일A34966
2355157Certificate of Analysis2021년 10월 06일A34966
시험 성적서 관련 5 개의 결과가 검색되었습니다.
Safety Data Sheets | 물질 안전 보건 자료
SDSScientific Resources
Product Information
라벨 라이센스 사용이 제한된 항목
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Notice to Purchaser: this product can only be purchased by non-profit purchasers.
Non-Profit Purchaser. The purchase of this product by a Non-Profit Purchaser conveys to the purchaser the limited, non-transferable right to use the product only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in commercial applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of purchaser’s activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
Commercial Purchaser. Commercial Purchasers have no rights to use the product are conveyed to purchaser. Such rights may be obtained through a separate written agreement between purchaser and Thermo Fisher Scientific. For information on obtaining a license, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States. If purchaser does not accept these terms, purchaser may return the products to Thermo Fisher for a refund.
"Commercial Purchaser" means any entity or organization other than a Non-Profit Purchaser.
"Non-Profit Purchaser" means any college, university or governmental entity (including without limitation, governmental and quasi-governmental institutes and research laboratories), or any non-profit scientific, research or educational organization of the type described in section 501(c)(3) of the Internal Revenue Code or any foreign equivalent regulation, or qualified under a state non-profit organization statute.
자주 묻는 질문(FAQ)
Either method will work in arresting cell division. However, the irradiation process will ensure that cell division will cease regardless of cell aggregation. Cell clumping can potentially not inactivate all cells when using mitomycin C, as cells within clumps may not be exposed to the mitomycin C. Irradiated cells are preferred by those who have concerns about chemical treatment. Mitomycin C-treated cells are preferred by those who have concerns about DNA damage from irradiation.
CF1 mouse embryonic fibroblasts do not have drug resistance. CF6 mouse embryonic fibroblasts are resistant to Neomycin/geneticin (G418). DR 4 mouse embryonic fibroblasts are resistant to geneticin (G418), puromycin, hygromycin, and 6-thioguanine.
We recommend seeding these cells at densities ranging from 2 x 10E4 to 5.5 x 10E4 cells/cm2. A good starting point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be lost.
These cells should be plated 24 hours prior to plating the ESCs or iPSCs and should be used for only 7-10 days.
Yes, the culture vessel needs to be coated with Attachment Factor protein (Cat. No. S006100) at 37 degrees C for 30 mins or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.