Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Certificates
SDS
Citations & References (2)
Gibco™
RPMI 1640 Medium, powder, HEPES
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
PromotionPromo code:RCELL25
Save up to 29% across the cell culture workflow - research begins with cells
From now until Dec 31, get up to 29% off media, sera, buffers, reagents and other cell culture products to advance your research - spend $800 and get 22% off or spend $1300 and get 29% offLearn More
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| 23400062 | 10 L |
| 23400021 | 10 x 1 L |
Catalog number 23400062
Price (TWD)
2,570.00
Online offer
Ends: 30-Sep-2025
3,670.00Save 1,100.00 (30%)
Each
In stock
Quantity:
10 L
Price (TWD)
2,570.00
Online offer
Ends: 30-Sep-2025
3,670.00Save 1,100.00 (30%)
Each
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • Phenol Red | |
| • HEPES |
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity10 L
Shelf Life36 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormPowder
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red
Without AdditivesNo Sodium Pyruvate, No Sodium Bicarbonate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Media Formulations
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Frequently asked questions (FAQs)
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3066232Certificate of AnalysisJun 28, 202523400021, 23400062
3124517Certificate of AnalysisJun 18, 202523400021, 23400062
3066231Certificate of AnalysisMay 14, 202523400021, 23400062
3066230Certificate of AnalysisFeb 09, 202523400021, 23400062
2522452Certificate of AnalysisJan 29, 202523400021, 23400062
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSCitations & References (2)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.
Journal:J Biol Chem
PubMed ID:12082101
'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study,
Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.
Journal:J Biol Chem
PubMed ID:12084719
Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in growth and survival of carcinomas. In this study, LPA production and response were characterized in two human prostate cancer (CaP) cell lines: PC-3 and Du145. Bombesin, a neuroendocrine peptide that is mitogenic for CaP cells, stimulated
2 total citations