Spectra™ Multicolor High Range Protein Ladder
Spectra™ Multicolor High Range Protein Ladder
Thermo Scientific™

Spectra™ Multicolor High Range Protein Ladder

Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to深入閱讀
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產品號碼Quantity
266252 x 250 μL
26625X48 x 250 μL
產品號碼 26625
價格 (TWD)
-
Quantity:
2 x 250 μL
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Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Compare and view all other protein standards and ladders ›

Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

For Research Use Only. Not for use in diagnostic procedures.
規格
Detection MethodColorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)300, 250, 180, 130, 100, 70, 50, 40 kDa
Product LineSpectra™
Product TypeProtein Ladder
Quantity2 x 250 μL
Ready to LoadYes
Recommended ApplicationsTris-acetate gels
Shipping ConditionApproved for shipment on Wet or Dry Ice
Stain Type3 colors: Blue, Orange, Green
System TypeWestern Blotting, SDS-PAGE
Number of Markers8
Size Range40 to 300 kDa
Unit SizeEach
內容物與存放
Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C

iBlot 3 Western Blot Transfer System

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批號Certificate TypeDateCatalog Number(s)
3260043Certificate of AnalysisJun 12, 202526625
3254769Certificate of AnalysisJun 05, 202526625
3234017Certificate of AnalysisMay 07, 202526625
3195771Certificate of AnalysisMar 24, 202526625
2801605Certificate of AnalysisJan 29, 202526625
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安全資料表

常見問答集 (常見問題)

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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