NuPAGE™ MOPS SDS Running Buffer (20X)
NuPAGE™ MOPS SDS Running Buffer (20X)
Invitrogen™

NuPAGE™ MOPS SDS Running Buffer (20X)

NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating深入閱讀
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產品號碼Quantity
NP0001500 mL
NP0001025 L
產品號碼 NP0001
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Quantity:
500 mL
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NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
規格
Chemical Name or MaterialRunning Buffer
Formulation50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide
Recommended StorageContents: NuPAGE™ MOPS SDS Running Buffer (20X)
Storage: +4°C to 25°C
Concentration20X
Physical FormLiquid
Product LineNuPAGE
Quantity500 mL
pH7.7
Unit SizeEach
iBlot 3 Western Blot Transfer System

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
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批號Certificate TypeDateCatalog Number(s)
3178557Certificate of AnalysisJun 24, 2025NP000102
3192257Certificate of AnalysisJun 16, 2025NP0001
3163044Certificate of AnalysisMay 22, 2025NP0001
3141691Certificate of AnalysisApr 17, 2025NP0001
3136368Certificate of AnalysisApr 11, 2025NP000102
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安全資料表

常見問答集 (常見問題)

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

We do not recommend using NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions. This buffer system may generate excessive heat, resulting in poor band resolution. Further, the protein of interest may not migrate very well in a neutral pH environment if it is not charged.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

引用資料與參考文獻 (4)

引用資料與參考文獻
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ,Smith KC,Chalberg C,Truong C,Burton DW,Deftos LJ,Bouvet M
Journal:Endocrinology
PubMed ID:15878959
The importance of an innervated and intact antrum and pylorus in preventing postoperative duodenogastric reflux and gastritis.
Authors:Keighley MR,Asquith P,Edwards JA,Alexander-Williams J
Journal:The British journal of surgery
PubMed ID:123
Molecular characterization of a metastatic neuroendocrine cell cancer arising in the prostates of transgenic mice.
Authors:Hu Y, Ippolito JE, Garabedian EM, Humphrey PA, Gordon JI,
Journal:J Biol Chem
PubMed ID:12228243
The features and functions of prostatic neuroendocrine (NE) cells remain ill-defined. Neuroendocrine differentiation (NED) in adenocarcinoma of the human prostate (CaP) is associated with more aggressive disease, but the underlying mediators are poorly understood. We examined these issues in transgenic mice that utilize regulatory elements from the cryptdin-2 gene (Defcr2) ... More
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More
4 total citations

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