Conceptual art of a One Shot FBS bottle and packaging over a cell

The One Shot FBS: Fetal bovine serum in convenient 50 mL bottles

Gibco One Shot FBS is an aliquot-free solution for serum use, minimizing contamination and saving time. The One Shot FBS 50 mL bottle is designed to eliminate a tedious lab task and significantly improve your cell culture results.

Videos: Gibco One Shot FBS eliminates the need to aliquot FBS

The new Gibco™ One Shot™ FBS 50 mL bottle. Finally…Aliquot-free solution that reduces contamination.
If you’re one of the more than 80% of researchers who aliquots their FBS into 50 mL conical tubes, your life is about to change for the better. Pour and pipette with the greatest of ease.
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        1. Now Playing
          Up NextThe new Gibco™ One Shot™ FBS 50 mL bottle. Finally…Aliquot-free solution that reduces contamination.

          If you’re one of the more than 80% of researchers who aliquots their FBS into 50 mL conical tubes, your life is about to change for the better. Pour and pipette with the greatest of ease.

        2. Now Playing
          Up NextGibco One Shot FBS 50 mL bottle: Customer Testimonial

          Learn more at thermofisher.com/oneshot Hear what Dr. Christian is saying about the Gibco One Shot FBS 50 mL bottle. Saving time in the lab and reducing the risk of contamination with this aliquot-free solution is a great thing.

        3. Now Playing
          Up NextGibco One Shot FBS 50 mL bottle: How to properly aliquot and heat inactivate your FBS

          Learn more at thermofisher.com/oneshot. Let our scientist show you how to properly aliquot your FBS into 50 mL conical tubes as well as heat inactivate the FBS to ensure an optimal outcome.

        4. Now Playing
          Up NextIs there a way around the frustration of aliquoting many bottles of serum?

          Learn more at thermofisher.com/oneshot Two researchers from neighboring cell culture labs discuss the frustation of aliquoting many bottles of serum into 50 mL conical tubes.

        5. Now Playing
          Up NextHow can the Gibco One Shot 50 mL bottle reduce your lab's plastic waste by as much as 33%?

          Learn more at thermofisher.com/oneshot Our researchers discuss how a unique shaped bottle can help reduce plastic waste in lab. Not only that, it will remove the need to aliquot your serum.

      Streamline your serum prep with One Shot FBS

      Researcher quote

      “This bottle allows for convenience of speed while maintaining a sterile product. As we have lots of undergrads in and out of the lab, this ensures that one mishap with not-so-aged hands doesn't ruin a whole pack of FBS.”

      Bethany C. Prudner, PhD, Metabolomic Sarcoma Lab, Washington University in St. Louis

      Explore the many benefits of One Shot FBS

      Bottle of One Shot FBS with other FBS container types in the background

      End the need to aliquot FBS

      Aliquoting FBS into 50 mL conical tubes increases the chances of compromising your results. The One Shot FBS 50 mL bottle’s size and design eliminates your need to aliquot FBS, putting you in control of your time, contamination risk, and volume consistency.

      Pipette drawing FBS from a One Shot FBS 50 mL bottle

      Pour and pipet with ease

      The bottle’s wide mouth and chamfered edge enable smooth, burp-free pouring. In addition, whether pouring or using a 25 or 50 mL pipette, you’ll be able to get every last drop.

      One Shot FBS bottles placed in a rack that is soaking in a water bath

      Stop the freeze-thaw-repeat cycle

      Skipping the FBS aliquot step means you only have to freeze then thaw your serum once—saving considerable time and effort. Because the bottles are engineered to withstand freezing, they won’t crack and pop their lids like conical tubes often do.

      Labeling on a One Shot FBS bottle versus a traditional conical tube

      End labeling mishaps

      Missing SKU or lot numbers, expiration dates, origin—or even the entire label—can cause a chain reaction of problems. The One Shot bottle has an easy-to-read package label, so you know exactly what you’re using.

      One Shot FBS package sitting in a lab freezer next to other types of reagents

      Free up freezer space

      The One Shot FBS package design is optimized to ease the burden of FBS storage on your freezer capacity, improving FBS storage in your lab. In fact, the One Shot FBS 10 x 50 mL carton allows you to store up 57 L more than our 100 mL bottle.

      Nine One Shot FBS bottles sitting in a blue test tube rack

      Pack the rack for convenient FBS storage

      Your test tube racks aren’t just for conical tubes anymore. The One Shot FBS 50 mL bottle fits in most test tube racks—including strong, durable 30 mm Nalgene Unwire Racks. So, you get the convenience of fit in an easy-pour, easy-store, aliquot-free bottle.

      A variety of plastic consumables including 500 mL FBS bottle, ten conical tubes, and a plastic serological pipette

      Create less waste by eliminating FBS aliquots

      The One Shot FBS 50 mL bottle generates 33% less waste than aliquoting from 500 mL bottles (Table 1). By using less plastic, less petroleum feedstock is consumed, and fewer greenhouse gas emissions are generated. By choosing One Shot FBS, a typical customer using 20 L of FBS per year would gain almost 7 hours back and generate 7 lb less plastic waste. This is a significant benefit both to lab productivity and the environment.

      Table 1. Waste reduction using One Shot FBS 50 mL bottles versus aliquoting from 500 mL bottle
      MethodStep in procedurePlastic requiredWeight (g)Total plastic weight (g)
      Aliquot from 500 mL bottleOriginal container1 x 500 mL bottle79.4 
      Aliquot to conicals1 x 25 mL serological pipette18.8 
      10 conical tubes140.3238.5
      One Shot FBS 50 mL BottlesOriginal container10 x 50 mL bottles159.0159.0
       33% waste reduction

       

      One Shot FBS bottle sitting between a bottle of Gibco DMEM and a bottle of Gibco RPMI

      Pair for best performance

      The 50 mL bottle size is a perfect match for use with a 500 mL bottle of Gibco RPMI, DMEM, or other Gibco cell culture media. This complete media pairing can help maximize performance and reproducibility.

      For questions about the One Shot FBS 50 mL bottle's specifications, contact technical services at techsupport@thermofisher.com.

      Single 50 mL bottle of One Shot FBS and a One Shot 10 x 50 mL carton

      Available in single 50 mL bottle or 10-bottle pack

      Choose a single 50 mL bottle or the One Shot carton, with a configuration of 10 x 50 mL.


      Order One Shot FBS

      One Shot FBS

      Select the most cited FBS products in global scientific journals, packaged in convenient One Shot bottles.

      Catalog # Name Size Price (USD) Qty
      A5670401 Fetal Bovine Serum (FBS), Premium, One Shot™ format, 50 mL Each
      95.65

      Online Exclusive

      105.00
      Save 9.35 (9%)
      A5669401 Fetal Bovine Serum, Premium Plus, One Shot™ format, 50 mL Each
      112.65

      Online Exclusive

      126.00
      Save 13.35 (11%)
      A5670402 Fetal Bovine Serum (FBS), Premium, One Shot™ format, 10 x 50 mL Each
      866.65

      Online Exclusive

      992.00
      Save 125.35 (13%)
      A5669402 Fetal Bovine Serum, Premium Plus, One Shot™ format, 10 x 50 mL Each
      1,049.65

      Online Exclusive

      1,184.00
      Save 134.35 (11%)
      A5209401 Fetal Bovine Serum, Value, One Shot™ format, 50 mL Each
      61.65

      Online Exclusive

      71.50
      Save 9.85 (14%)
      A5209402 Fetal Bovine Serum, Value, One Shot™ format, 10 x 50 mL Each
      572.65

      Online Exclusive

      676.00
      Save 103.35 (15%)

      Heat-inactivated (HI) FBS

      Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.

      Catalog # Name Size Price (USD) Qty
      A5670501 Fetal Bovine Serum (FBS), Premium, heat-inactivated, One Shot™ format, 50 mL Each
      101.65

      Online Exclusive

      113.00
      Save 11.35 (10%)
      A5669501 Fetal Bovine Serum, Premium Plus, heat inactivated, One Shot™ format, 50 mL Each
      120.65

      Online Exclusive

      135.00
      Save 14.35 (11%)
      A5669502 Fetal Bovine Serum, Premium Plus, heat inactivated, One Shot™ format, 10 x 50 mL Each
      1,125.65

      Online Exclusive

      1,268.00
      Save 142.35 (11%)
      A5670502 Fetal Bovine Serum (FBS), Premium, heat-inactivated, One Shot™ format, 10 x 50 mL Each
      943.65

      Online Exclusive

      1,060.00
      Save 116.35 (11%)
      A5209501 Fetal Bovine Serum, Value, heat inactivated, One Shot™ format, 50 mL Each
      70.65

      Online Exclusive

      76.50
      Save 5.85 (8%)
      A5209502 Fetal Bovine Serum, Value, heat inactivated, One Shot™ format, 10 x 50 mL Each
      663.65

      Online Exclusive

      722.00
      Save 58.35 (8%)

      Learn more about our HI products at thermofisher.com/hifbs

      Specialty FBS

      Select Specialty One Shot FBS qualified for specific research applications and assays.

      Learn more about our specialty FBS products at thermofisher.com/specialtyfbs

      One Shot FBS

      Select the most cited FBS products in global scientific journals, packaged in convenient One Shot bottles.

      Heat-inactivated (HI) FBS

      Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.

      Learn more about our HI products at thermofisher.com/hifbs

      Specialty FBS

      Select Specialty One Shot FBS qualified for specific research applications and assays.

      Learn more about our specialty FBS products at thermofisher.com/specialtyfbs

      Resources

      Fetal Bovine Serum Basics
      Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.

      Cell Culture Basics
      Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.

      Gibco iMatch Sera Lot Matching Tool 
      Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.

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      For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.

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