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Catalog Number | Quantity | Format | Heat Inactivated | Gamma-irradiated |
---|---|---|---|---|
A5670501 | 50 mL | One Shot | Yes | No |
A5670401 | 50 mL | One Shot | No | No |
A5670402 | 10 x 50 mL | One Shot | No | No |
A5670502 | 10 x 50 mL | One Shot | Yes | No |
A5670701 | 500 mL | Bottle | No | No |
A5670801 | 500 mL | Bottle | Yes | No |
A5670901 | 500 mL | Bottle | No | Yes |
A5670201 | 500 mL | Bottle | Yes | Yes |
Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.
Sera category: Premium
Gibco serum delivers
Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.
1. Pellerin, et al., Bioengineering, published in 2021.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Our CoAs only capture the total protein content not specifically the amount attributed to albumin.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
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