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Super Bright 600 Dye
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| 5 | 405 | 610/20 | 414 | 601 | (in buffer) 5 | flow cytometry |
Invitrogen eBioscience Super Bright 600 is tandem polymer dye, consisting of violet (405 nm) laser–excitable Super Bright 436 and an acceptor dye that emits fluorescence signal at 600 nm. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry because they emit fluorescence with high efficacy. Super Bright 600 antibody conjugates are an alternative to Brilliant Violet 605 conjugates, offering increased resolution of positive and negative populations with less background and less interfering dye-dye interactions.
Super Bright conjugates are fully compatible with UltraComp eBeads microspheres for compensation, viability stains, fluorescent dyes and other reagents commonly used in flow cytometry. Robust, highly sensitive, and stable, Super Bright conjugates easily fit into any flow cytometry panel. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer Super Bright 600 dye conjugated to primary antibodies and streptavidin for use in flow cytometry.
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Additional products
Staining of normal canine peripheral blood cells with Super Bright 600 conjugated antibody. Normal canine peripheral blood cells (PBMCs) were stained with anti-Canine CD4 Monoclonal Antibody (YKIX302.9), FITC and Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 600 (left) or anti-Canine CD25 Monoclonal Antibody (P4A10), Super Bright 600 (right). Viable cells, determined by 7-AAD Viability Staining Solution were used for analysis.
Super Bright dyes
Super Bright polymer dyes are violet laser–excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals. The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.