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PE-Cyanine7 Dye
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| 4 | 488, 532, 561 | 780/60 | 569 | 780 | (in buffer) 1 | flow cytometry |
Invitrogen PE-Cyanine7 is a bright tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye degradation is common if exposed to photobleaching, fixation, and higher temperatures. PE-Cy7 is a near-IR dye used primarily in flow cytometry for low-abundance antigens.
We offer PE-Cyanine7 dye conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection.
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Comparison of fluorescence intensity of PE-Cyanine7 conjugated antibodies in human PBMCs. Normal human peripheral blood cells (PBMCs) were stained with Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7 (blue histogram) or CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.
Cell surface staining of human PBMCs using PE-Cyanine7. Normal human peripheral blood cells (PBMCs) were stained with CD3 Monoclonal Antibody (UCHT1), APC, TCR gamma/delta Monoclonal Antibody (B1.1), PerCP-eFluor 710 and Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7(left) or TCR V delta 1 Monoclonal Antibody (TS8.2), PE-Cyanine7 (right). Viable CD3 positive lymphocytes were used for analysis.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
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