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Gibco™
MEM
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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| Catalog Number | Quantity |
|---|---|
| 11095080 | 500 mL |
| 11095098 | 10 x 500 mL |
| 11095072 | 1000 mL |
| 11095114 | 6 x 1000 mL |
Catalog number 11095080
Price (USD)
34.65
Online Exclusive
37.45Save 2.80 (7%)
Each
In stock
Quantity:
500 mL
Price (USD)
34.65
Online Exclusive
37.45Save 2.80 (7%)
Each
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Figures
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Cellular viability and proliferation in presence of ProLong® Live Antifade Reagent
HeLa cells transduced with Organelle Lights™ Mito-GFP.
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Lot #Certificate TypeDateCatalog Number(s)
3140517Certificate of AnalysisJul 12, 202511095072, 11095080
3166041Certificate of AnalysisJul 03, 202511095072, 11095080
3148666Certificate of AnalysisJun 28, 202511095072, 11095080
3224811Certificate of AnalysisJun 19, 202511095072, 11095080
3138471Certificate of AnalysisMay 03, 202511095072, 11095080
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SDSCitations & References (10)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Influx of calcium through a redox-sensitive plasma membrane channel in thymocytes causes early necrotic cell death induced by the epipolythiodioxopiperazine toxins.
Journal:J Biol Chem
PubMed ID:12063251
'Gliotoxin, a member of the epipolythiodioxopiperazine (ETP) class of toxins, induces both apoptotic and necrotic cell death in a concentration-dependent manner. Whereas the specific trigger for apoptotic death caused by these toxins is unclear, the reactive disulfide bond in the ETP toxins is required for biological activity. Thus it is
Two adaptor proteins differentially modulate the phosphorylation and biophysics of kv1.3 ion channel by SRC kinase.
Journal:J Biol Chem
PubMed ID:11812778
'The Shaker family K(+) channel protein, Kv1.3, is tyrosine phosphorylated by v-Src kinase at Tyr(137) and Tyr(449) to modulate current magnitude and kinetic properties. Despite two proline rich sequences and these phosphotyrosines contained in the carboxyl and amino terminals of the channel, v-Src kinase fails to co-immunoprecipitate with Kv1.3 as
Antagonism between Ena/VASP Proteins and Actin Filament Capping Regulates Fibroblast Motility.
Journal:Cell
PubMed ID:12086607
'Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP proteins accumulate in protruding lamellipodia and promote the rapid actin-driven motility of the pathogen Listeria. In contrast, Ena/VASP negatively regulate cell translocation. To resolve this paradox, we analyzed the function of Ena/VASP during lamellipodial protrusion. Ena/VASP-deficient lamellipodia protruded
A serum- and antioxidant-free primary culture model of mouse cortical neurons for pharmacological screen and studies of neurotrophic and neuroprotective agents.
Journal:Cell Mol Neurobiol
PubMed ID:12363202
'1. Morphologically developmental properties of fetal mouse cortical neurons in the chemically defined serum- and antioxidant-free culture condition were observed. Also, cellular composition in cultures was identified by immunostaining with anti-NSE and anti-GFAP. 2. Various cell densities ranging from 1 x 10(3) to 1 x 10(6) cells/cm2 were prepared to
Dependence of selective gene activation on the androgen receptor NH2- and COOH-terminal interaction.
Journal:J Biol Chem
PubMed ID:12000757
'The agonist-induced androgen receptor NH(2)- and COOH-terminal (N/C) interaction is mediated by the FXXLF and WXXLF NH(2)-terminal motifs. Here we demonstrate that agonist-dependent transactivation of prostate-specific antigen (PSA) and probasin enhancer/promoter regions requires the N/C interaction, whereas the sex-limited protein gene and mouse mammary tumor virus long terminal repeat do
10 total citations