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Lipofectamine™ 2000 Transfection Reagent
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety ofRead more
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| Catalog Number | Quantity |
|---|---|
| 11668027 | 0.75 mL |
| 11668019 | 1.5 mL |
| 11668500 | 15 mL |
| 11668030 | 0.3 mL |
Catalog number 11668027
Price (USD)
519.20
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590.00Save 70.80 (12%)
Each
In stock
Quantity:
0.75 mL
Price (USD)
519.20
Special offer
Online exclusive
590.00Save 70.80 (12%)
Each
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine™ 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
Green FeaturesSustainable packaging
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Contains one vial (0.75 ml) Lipofectamine™ 2000 Reagent. Store at 2–8°C. Do not freeze.
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Figures
Lipofectamine® 2000 transfection example
Lipofectamine™ 2000 cell line performance
Plasmid transfection
GFP expression
Lipofectamine® 2000 transfection protocol
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Lot #Certificate TypeDateCatalog Number(s)
3141896Certificate of AnalysisMay 21, 202511668019
3105258Certificate of AnalysisApr 28, 202511668019
3100038Certificate of AnalysisApr 28, 202511668030
3071541Certificate of AnalysisApr 25, 202511668027
3141899Certificate of AnalysisApr 15, 202511668500
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Safety Data Sheets
SDSScientific Resources
Product Information
Protocols
Frequently asked questions (FAQs)
Transfection with cationic lipids can produce light granular orange background fluorescence. The orange fluorescence is associated with the lipid/DNA complexes and is not related to GFP. This background varies depending on the cationic lipid reagent used and does not interfere with transfection results. If desired, try performing fluorescence imaging in PBS instead of culture medium. Also, make sure the cells are healthy and intact as lysed cells or cells under stress could generate autofluorescence products.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Yes, all of our lipid transfection reagents are stable at room temperature for months.
Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.
While Lipofectamine 3000 or Lipofectamine 2000 may be used for co-transfection of siRNA with plasmid DNA, Lipofectamine RNAiMAX cannot be used.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
You can co-transfect plasmids and siRNA using Lipofectamine 2000. Please click here (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/transfection-protocol/plasmid-co-transfection-protocol-lipofectamine-sirna.html) for protocol information.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Lipofectamine 2000:DNA complexes are stable for 6 hours at room temperature when the complex is prepared in Opti-MEM medium. Use of other media for complex formation may decrease stability.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Citations & References (281)
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Citations & References
Abstract
Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16611727
Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal
Characterization of aquaporin-6 as a nitrate channel in mammalian cells. Requirement of pore-lining residue threonine 63.
Journal:J Biol Chem
PubMed ID:12177001
Aquaporins (AQP) were originally regarded as plasma membrane channels that are freely permeated by water or small uncharged solutes but not by ions. Unlike other aquaporins, AQP6 overexpressed in Xenopus laevis oocytes was previously found to exhibit Hg2+ or pH-activated ion conductance. AQP6 could not be analyzed electrophysiologically in mammalian
Role of tyrosine kinase Jak2 in prolactin-induced differentiation and growth of mammary epithelial cells.
Journal:J Biol Chem
PubMed ID:11821424
Genetic studies in mice have established a critical role for prolactin receptors and transcription factor Stat5 in mammary gland differentiation. However, the enzymatic coupling between prolactin receptors and Stat5 in this process has not been established. In addition to Jak2, several other tyrosine kinases reportedly also are associated with prolactin
Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12409609
When cells are exposed to death-inducing molecules such as tumor necrosis factor-alpha or Fas, caspase 8 is activated and cleaves an apoptotic facilitator, Bid, that is a member of the Bcl-2 family. After additional modification, the C-terminal moiety of Bid is translocated to the mitochondria and induces the release of
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T
281 total citations