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RPMI 1640 Medium
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park MemorialRead more
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| Catalog Number | Quantity |
|---|---|
| 11875168 | 5 L |
| 11875176 | 10 L |
Catalog number 11875168
Price (USD)
415.00
Each
In stock
Quantity:
5 L
Price (USD)
415.00
Each
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, SynerGy™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity5 L
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesGlutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3247042Certificate of AnalysisJun 27, 202511875176, 11875101, 11875168, 11875085, 11875093
3247040Certificate of AnalysisJun 26, 202511875176, 11875101, 11875168, 11875085, 11875093
3247038Certificate of AnalysisJun 26, 202511875176, 11875101, 11875168, 11875085, 11875093
3247036Certificate of AnalysisJun 26, 202511875176, 11875101, 11875168, 11875085, 11875093
3175947Certificate of AnalysisJun 22, 202511875176, 11875101, 11875168, 11875085, 11875093
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.