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Gibco™
RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension andRead more
| Catalog Number | Quantity |
|---|---|
| 72400047 | 500 mL |
Catalog number 72400047
Price (EUR)
73,00
Each
-
Quantity:
500 mL
Price (EUR)
73,00
Each
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
| With |
| • GlutaMAX™ |
| • Phenol Red |
| • HEPES |
The complete formulation is available.
Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, GlutaMAX™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutaMAX, HEPES, Phenol Red
Without AdditivesNo Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Media Formulations
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Figures
L-glutamine is prone to spontaneous degradation
L-glutamine degrades faster than GlutaMAX Supplement
GlutaMAX Supplement delivers L-glutamine with more stability
GlutaMAX Supplement reduces ammonia production from L-glutamine
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3141660Certificate of AnalysisJun 27, 202572400047, 72400146, 72400013, 72400021, 72400153
3221713Certificate of AnalysisJun 14, 202572400047, 72400146, 72400013, 72400021, 72400153
3209607Certificate of AnalysisJun 13, 202572400047, 72400146, 72400013, 72400021, 72400153
3126851Certificate of AnalysisJun 05, 202572400047, 72400146, 72400013, 72400021, 72400153
3224718Certificate of AnalysisMay 31, 202572400047, 72400146, 72400013, 72400021, 72400153
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (3)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis.
Journal:J Biol Chem
PubMed ID:11294857
'Hypoxia is a common environmental stress that influences signaling pathways and cell function. Several cell types, including neuroendocrine chromaffin cells, have evolved to sense oxygen levels and initiate specific adaptive responses to hypoxia. Here we report that under hypoxic conditions, rat pheochromocytoma PC12 cells are resistant to apoptosis induced by
Evidence for the involvement of diacylglycerol kinase in the activation of hypoxia-inducible transcription factor 1 by low oxygen tension.
Journal:J Biol Chem
PubMed ID:11136721
Hypoxia-inducible factor 1 (HIF-1) induces a gene expression program essential for the cellular adaptation to lowered oxygen environments. The intracellular mechanisms by which hypoxia induces HIF-1 remain poorly understood. Here we show that exposure of various cell types to hypoxia raises the intracellular level of phosphatidic acid primarily through the
Chemical synthesis and biological activity of bromohydrin pyrophosphate, a potent stimulator of human gamma delta T cells.
Journal:J Biol Chem
PubMed ID:11279081
Small phosphorylated metabolites from mycobacteria stimulate human gammadelta T lymphocytes. Although such phosphoantigens could prove useful in the composition of vaccines involving gammadelta T cell-mediated immunity, their very low abundance in natural sources limits such applications. Here, we describe the chemical production, purification, and bioactivity of a phosphorylated bromohydrin (BrHPP)
3 total citations