William's E Medium, no glutamine

This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

Gibco™

William's E Medium, no glutamine

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells.

Catalog Number	Quantity
12551032	500 mL

Catalog number 12551032

Price (USD)

56.65

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62.50

Save 5.85 (9%)

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Quantity:

500 mL

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Price (USD)

56.65

Online Exclusive

62.50

Save 5.85 (9%)

Add to cart

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

This William's E Medium is modified as follows:

With: Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Dual-Site cGMP Manufacturing

Gibco™ William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

For Use With (Application)Hepatocytes and hepatic models, mammalian cell culture, pharma and biopharma, primary cell culture

Product TypeWilliam's E Medium

Quantity500 mL

Shelf Life12 Months

Shipping ConditionRoom Temperature

Culture TypeMammalian Cell Culture

FormLiquid

With AdditivesPhenol Red, Sodium Pyruvate

Without AdditivesNo Glutamine, No HEPES

Unit SizeEach

Contents & Storage

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all William's E Medium, no glutamine FAQs