Nunc™ Lab-Tek™ Chambered Coverglass
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Nunc™ Lab-Tek™ Chambered Coverglass
Thermo Scientific™

Nunc™ Lab-Tek™ Chambered Coverglass

Perform high-magnification microscopy and confocal image analysis with Thermo Scientific™ Nunc™ Lab-Tek™ Chambered Coverglass.
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Catalog NumberWellsMaterialDescriptionUnit Size
1553611Borosilicate Glass (1.0)1-well Chambered Coverglass w/ non-removable wellCase of 96
155361PK
also known as 155361
1Borosilicate Glass (1.0)1-well Chambered Coverglass w/ non-removable wellPack of 16
1553802Borosilicate Glass (1.0)2-well Chambered Coverglass w/ non-removable wellsCase of 96
155380PK
also known as 155380
2Borosilicate Glass (1.0)2-well Chambered Coverglass w/ non-removable wellsPack of 16
1553834Borosilicate Glass (1.0)4-well Chambered Coverglass w/ non-removable wellsCase of 96
155383PK
also known as 155383
4Borosilicate Glass (1.0)4-well Chambered Coverglass w/ non-removable wellsPack of 16
1554118Borosilicate Glass (1.0)8-well Chambered Coverglass w/ non-removable wellsCase of 96
155411PK8Borosilicate Glass (1.0)8-well Chambered Coverglass w/ non-removable wellsPack of 16
Catalog number 155361
Price (USD)
1,306.00
Case of 96
In stock
Add to cart
Wells:
1
Material:
Borosilicate Glass (1.0)
Description:
1-well Chambered Coverglass w/ non-removable well
Unit Size:
Case of 96
Recurring order eligible. Learn more »
Price (USD)
1,306.00
Case of 96
Add to cart
Ask our AI about this Product
Grow, stain and view living cells on a single device. Thermo Scientific™ Nunc™ Lab-Tek™ Chambered Coverglass with a No. 1 borosilicate glass bottom offers the optical characteristics required for high magnification and confocal image analysis. Available in 1-, 2-, 4-, and 8-well formats. The upper chamber structure cannot be removed from the coverglass.
Features:
  • Media chamber securely mounted to No. 1 borosilicate coverglass by non-toxic medical grade silicone adhesive
  • The polystyrene chamber is not removable and requires inverted microscope for viewing
  • Fitted polystyrene cover without top handle minimizes evaporation

Ordering Information:

  • Can be purchased in case of 96 or pack of 16.
  • Shipped sterile in convenient stackable incubation trays.


Warranty: 90 days

 

WARNING: Cancer – www.P65Warnings.ca.gov
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Closure ColorClear
Description1-well Chambered Coverglass w/ non-removable well
IncludesClear polystyrene lid with handle on the side for aseptic manipulations
Lid MaterialPolystyrene
No. per Pack16
Culture Area9.4 cm2
Dimensions (LxW)57 x 25 mm
MaterialBorosilicate Glass (1.0)
SterilitySterile
Surface TreatmentCell Culture
Thickness (Metric)0.13 to 0.17 mm
Volume (Metric) Working2.5 to 4.5 mL
Wells1
Unit SizeCase of 96
ECM
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Lot #Certificate TypeDateCatalog Number(s)
1271900Certificate of ComplianceDec 11, 2024155380
1289427Certificate of ComplianceDec 11, 2024155383
1411260Certificate of ComplianceNov 28, 2024155411
1408086Certificate of ComplianceJun 12, 2024155411
1407817Certificate of ComplianceJun 04, 2024155380
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Frequently asked questions (FAQs)

The upper structure on our Nunc Lab-Tek Chambered Coverglass is not removable, and therefore not designed for coverslipping.

Please review the following possible causes and solutions:
- The microscope could be dirty from fingerprints or oils. We recommend cleaning your microscope prior to use and always after oil immersion. When using oil immersion, it is important to use the specific brand of oil designed and recommended for that particular microscope.
- An incorrect type of coverslip or coverglass can cause poor resolution. We recommend Nunc Lab-Tek Chambered Coverglass (made from 1.0 borosilicate glass) and Nunc Lab-Tek II Chambered Coverglass (made from 1.5 borosilicate glass) for the best resolution. Plastic coverslips may affect resolution at high magnification due to refractive index differences between the glass and plastic.
- An incorrect thickness for the coverglass can cause poor resolution. Please consult your microscope's manual for the best information.
- The coverslipping procedure may affect resolution. A clear seal is required, free of debris and particles. When performing oil immersion, bubbles may result in poor resolution under high magnification.

Lab-Tek and Lab-Tek II chambered coverglass products can be plated with cells, incubated, and handled in the same way. The two products differ in the thickness of the glass and the chamber size. Lab-Tek coverglass uses Borosilicate coverglass No. 1 that is 0.13 - 0.16 mm thick and Lab-Tek II coverglass uses Borosilicate coverglass No. 1.5 thath is 0.16 - 0.19 mm thick. Lab-Tek II coverglass chambers hold 10 - 50% more media than Lab-Tek coverglass chambers, depending on the format. Studies have shown that Lab-Tek II chambered coverglass works well with fastidious cells such as neuronal, as well as poorly adherent cell lines.

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

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