Fetal Bovine Serum, certified, United States

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*** NEW CATALOG NUMBER***

These products are being replaced with Premium Plus (FBS) performance-based products. The Premium Plus product specifications are improved and product stability, performance, manufacturing, storage, and transport processes are unchanged.

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Fetal Bovine Serum, certified, United States

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Citations & References (10)

Gibco™

Fetal Bovine Serum, certified, United States

Name: Fetal Bovine Serum, certified, United States
SKU: 16000044
Price: 1004.00 USD

Important Update: We've recently introduced Premium Plus Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Certified US origin, New Zealand origin, and Qualified Australia origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.

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Catalog Number	Quantity	Format
16000044	500 mL	Bottle
A3160401	50 mL	One Shot
A3160402	10 x 50 mL	One Shot
16000069	1000 mL	Bottle

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Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

Fetal Bovine Serum, Premium Plus, 500 mL bottle

Catalog number: A5669701

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Catalog number 16000044

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1,004.00

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Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Sera Category: Premium (Performance Plus)

Use for a broad range of cell types, especially sensitive cell lines
Our most characterized sera with the lowest endotoxin and hemoglobin levels
Endotoxin level: ≤5 EU/mL
Hemoglobin level: ≤15 mg/dL
Origin: United States

Gibco Serum Delivers

ISO 13485 certified, processed in FDA registered facilities
Triple filtered at 0.1 μm
Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Specifications

Endotoxin Concentration≤5 EU/mL

Hemoglobin Concentration≤15 mg/dL

Purity or Quality GradeCertified

Shipping ConditionFrozen

SpeciesCattle/Bovine

AgeFetal

Country of OriginUnited States

FormLiquid

FormatBottle

Product TypeFetal Bovine Serum

Quantity500 mL

Serum TreatmentStandard (Sterile-filtered)

SterilitySterile

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

Contents & Storage

Storage conditions: ≤-10°C
Shipping conditions: Frozen

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Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, certified, United States FAQs

Figures

Consistency of HEK293 Cell Growth in Gibco® FBS:

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Documents & Downloads

Certificates

Search by lot number or partial lot number

Lot #Certificate TypeDateCatalog Number(s)

U3223971PCertificate of AnalysisJul 02, 202516000069, 16000036, 16000044

U3187356PCertificate of AnalysisJun 15, 202516000069, 16000036, 16000044

U3250063RPCertificate of AnalysisJun 14, 202516000069, 16000036, 16000044

U3126596RPCertificate of AnalysisJun 06, 2025A3160402, A3160401

U3194011PCertificate of AnalysisMay 25, 202516000069, 16000036, 16000044

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Safety Data Sheets

SDS

Scientific Resources

One Shot FBS 50 mL bottles green fact sheet

Brochure: Gibco Sera Guide

Brochure: Gibco Cell Culture Sera for Bioprocessing

Brochure: One Shot FBS 50 mL Bottle

Flyer: Gibco FBS FAQs (frequently asked questions)

Green Features Fact Sheet: One Shot FBS 50 mL bottles

Citations & References (10)

Search citations by name, author, journal title or abstract text

Citations & References

Abstract

Fibroblast growth factor receptor-1 signaling induces osteopontin expression and vascular smooth muscle cell-dependent adventitial fibroblast migration in vitro.

Authors: Li Guohong; Oparil Suzanne; Kelpke Stacey S; Chen Yiu-Fai; Thompson John A;

Journal:Circulation

PubMed ID:12176960

'BACKGROUND: Increased expression of osteopontin (OPN), fibroblast growth factors (FGFs), and their type-1 receptor (FGFR-1) is associated with neointima formation and atherosclerosis. This study tested the hypothesis that ligand activation of FGFR-1 stimulates OPN expression in rat aortic smooth muscle cells (RASMCs), explored the signaling pathway involved, and assessed the ... More

Ultraviolet-induced junD activation and apoptosis in myeloblastic leukemia ML-1 cells.

Authors:Li T, Dai W, Lu L.

Journal:J Biol Chem

PubMed ID:12082101

'The exposure of mammalian cells to UV irradiation induces the expression of immediate early genes such as c-jun and c-fos and activates the transcription factors AP-1 and NF-kappaB. JunD is one of the three members of the Jun family and shares some functional characteristics with c-Jun. In the present study, ... More

Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.

Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;

Journal:Nat Med

PubMed ID:12118246

Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More

Ascorbic-acid transporter Slc23a1 is essential for vitamin C transport into the brain and for perinatal survival.

Authors: Sotiriou Sotiria; Gispert Suzana; Cheng Jun; Wang Yaohui; Chen Amy; Hoogstraten-Miller Shelley; Miller Georgina F; Kwon Oran; Levine Mark; Guttentag Susan H; Nussbaum Robert L;

Journal:Nat Med

PubMed ID:11984580

The only proven requirement for ascorbic acid (vitamin C) is in preventing scurvy, presumably because it is a cofactor for hydroxylases required for post-translational modifications that stabilize collagen. We have created mice deficient in the mouse ortholog (solute carrier family 23 member 1 or Slc23a1) of a rat ascorbic-acid transporter, ... More

Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device.

Authors: Yang Mengsu; Li Cheuk-Wing; Yang Jun;

Journal:Anal Chem

PubMed ID:12199565

We have developed a microfluidic device for on-chip monitoring of cellular reactions. The device consists of two primary analytical functions: control of cell transport and immobilization, and dilution of an analyte solution to generate a concentration gradient. In this device, a dam structure in parallel to the fluid flow was ... More

10 total citations