Suero fetal bovino, certificado, inactivado por calor, Estados Unidos

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Suero fetal bovino, certificado, inactivado por calor, Estados Unidos
Gibco™

Suero fetal bovino, certificado, inactivado por calor, Estados Unidos

Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidadFormato
10082147500 mLFrasco
A384000150 mLOne Shot
A384000210 x 50 mLOne Shot
Número de catálogo 10082147
Precio (EUR)
-
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Cantidad:
500 mL
Formato:
Frasco
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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto.

Categoría de los sueros: Prémium (rendimiento plus)
Origen: Estados Unidos
Nivel de endotoxinas: ≤5 EU/ml
Nivel de hemoglobina: ≤15 mg/dL
Para uso diagnóstico in vitro.
Especificaciones
Concentración de endotoxinas≤5 EU/mL
Concentración de hemoglobina≤15 mg/dL
Grado de pureza o calidadcontenedores
Condiciones de envíoCongelado
EspecieGanado/bovino
AgeFetal
País de origenEstados Unidos
FormularioLíquido
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad500 mL
Serum TreatmentInactivado por calor
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado
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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
U3262172RPCertificate of Analysis29 jun 202510082147
U3250072RPCertificate of Analysis20 jun 202510082147
U3178939PCertificate of Analysis10 may 202510082147
U3130132RPCertificate of Analysis15 mar 202510082147
U3126607RPCertificate of Analysis14 mar 2025A3840001, A3840002
Se muestran 5 resultados, busque arriba un certificado específico

Hojas de datos de seguridad

Preguntas frecuentes

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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