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Gibco™
Leibovitz's L-15 Medium, no phenol red
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.Read more
| Catalog Number | Quantity |
|---|---|
| 21083027 | 500 mL |
Catalog number 21083027
Price (USD)
72.65
Online Exclusive
77.25Save 4.60 (6%)
Each
-
Quantity:
500 mL
Price (USD)
72.65
Online Exclusive
77.25Save 4.60 (6%)
Each
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.
This L-15 is modified as follows:
| With | Without |
| • Galactose | • Glucose |
| • L-glutamine | • Phenol Red |
| • Sodium Pyruvate | • HEPES |
| • Sodium Bicarbonate |
The complete formulation is available.
Using L-15
L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Specifications
Cell LineHEP-2 monkey kidney cells
Cell Typeexplants of embryonic and adult human tissue
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeLeibovitz's L-15 Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
With AdditivesGlutamine, Sodium Pyruvate, Galactose
Without AdditivesNo Glucose, No HEPES, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3126879Certificate of AnalysisMay 03, 202521083027
3082985Certificate of AnalysisFeb 23, 202521083027
2323089Certificate of AnalysisJan 29, 202521083027
2451149Certificate of AnalysisJan 29, 202521083027
2766658Certificate of AnalysisJan 29, 202521083027
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Safety Data Sheets
SDSScientific Resources
Frequently asked questions (FAQs)
We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, Leibovitz L15 medium (liquid or powder) does not contain sodium bicarbonate (the formulation for each catalog number is published online). Developed for the support of cell growth in non CO2-equilibrated environments, L-15 formulations were developed without a sodium bicarbonate buffer system. L-15 medium is buffered by phosphates and free-base amino acids (L-arginine primarily, but also L-histidine and L-cysteine). Galactose and sodium pyruvate are used to replace glucose in order to reduce the production of acidic metabolites.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We offer the following Leibovitz media:
- Leibovitz's L-15 Medium, powder: Cat. Nos. 41300039 (10x 1L) and 41300070 (1x 10L)
- Leibovitz's L-15 Medium, no phenol red: Cat. No 21083027 (1x 500 mL)
- Leibovitz's L-15 Medium: Cat. Nos.11415064 (1x 500 mL) and 11415114 (10x 500 mL) (for sale in N. America, Latin America, and Asia-Pacific regions; Cat. No. 11415049 (for sale in Europe, Africa, and the Middle East
- Leibovitz's L-15 Medium with GlutaMAX Supplement: Cat. No. 31415029 (1x 500 mL)
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Citations & References (2)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Ancient origin of somatic and visceral neurons.
Journal:BMC Biol
PubMed ID:23631531
'A key to understanding the evolution of the nervous system on a large phylogenetic scale is the identification of homologous neuronal types. Here, we focus this search on the sensory and motor neurons of bilaterians, exploiting their well-defined molecular signatures in vertebrates. Sensorimotor circuits in vertebrates are of two types:
Antiapoptotic activity of the free caspase recruitment domain of procaspase-9: a novel endogenous rescue pathway in cell death.
Journal:J Biol Chem
PubMed ID:11825888
Mitochondrial injury initiates proteolytic processing of procaspase-9 into the large and small subunits, leading to apoptotic cell death. Here we show that the free caspase recruitment domain (CARD) released by procaspase-9 processing activates nuclear factor kappaB expression. A procaspase-9 construct with a point mutation that abrogates the release of the
2 total citations