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Pierce™ Biotinylated Protein Interaction Pull-Down Kit
The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of aRead more
| Catalog Number | Quantity |
|---|---|
| 21115 | 25 reactions kit |
Catalog number 21115
Price (USD)
686.00
Each
In stock
Quantity:
25 reactions kit
Price (USD)
686.00
Each
The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.
Features of the Biotinylated Protein Interaction Pull-Down Kit:
• Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
• Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
• Adaptable to single- or multiple-sample demands
• Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads
You provide the biotinylated protein as the 'bait' (see
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EZ-Link™ Desthiobiotinylation and Pull-Down Kit
Features of the Biotinylated Protein Interaction Pull-Down Kit:
• Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
• Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
• Adaptable to single- or multiple-sample demands
• Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads
You provide the biotinylated protein as the 'bait' (see
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EZ-Link™ Desthiobiotinylation and Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayAffinity Purification
For Use With (Application)Pull-Down (Biotin-Based)
For Use With (Equipment)Microcentrifuge
FormatKit
Quantity25 reactions kit
Sufficient For25 Reactions
TargetNot Target-Specific
Product LinePierce™
TypePull-Down Kit
Unit SizeEach
Contents & Storage
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Streptavidin Agarose Resin, 1.5 mL
• TBS Pack (makes 500mL), 1 pack
• Biotin Blocking Solution, 15 mL
• Wash Buffer (pH 5.0), 100 mL
• Elution Buffer (pH 2.8), 50 mL
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 200 tubes
Store at 4°C
• Streptavidin Agarose Resin, 1.5 mL
• TBS Pack (makes 500mL), 1 pack
• Biotin Blocking Solution, 15 mL
• Wash Buffer (pH 5.0), 100 mL
• Elution Buffer (pH 2.8), 50 mL
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 200 tubes
Store at 4°C
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Figures
Biotinylated α-Chymotrypsin (b-αCT) used to pull-down its interactor bovine pancreas trypsin inhibitor (BPTI)
Procedure summary for the Biotinylated Protein Interaction Pull-Down Kit
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Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
AB393789Certificate of AnalysisMar 21, 202521115
ZG387994Certificate of AnalysisAug 14, 202421115
ZA387993Certificate of AnalysisFeb 09, 202421115
ZA385146Certificate of AnalysisFeb 07, 202421115
YJ384112Certificate of AnalysisOct 18, 202321115
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Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.