Pierce™ IP Lysis Buffer
Pierce™ IP Lysis Buffer
Thermo Scientific™

Pierce™ IP Lysis Buffer

Thermo Scientific Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP)Read more
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Catalog NumberQuantity
87787100 mL
87788250 mL
Catalog number 87787
Price (USD)
114.65
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126.00
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Quantity:
100 mL
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Price (USD)
114.65
Online Exclusive
126.00
Save 11.35 (9%)
Each
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Thermo Scientific Pierce IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate.

Features of IP Lysis Buffer:

• Optimized for compatibility with immunoprecipitation and pull-down assays
• Compatible with protein assays, reporter assays and immunoassay procedures
• Ready-made formula is effective for extracting cytoplasmic, membrane and nuclear proteins
• Gentle formulation helps maintain protein complexes for co-immunoprecipitation
• Does not liberate genomic DNA which can cause high sample viscosity

IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer. Pierce IP Lysis Buffer is specially formulated for pull-down and immunoprecipitation assays.

Pierce IP Lysis Buffer is effective for lysing cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. Optimized for pull-down and immunoprecipitation assays, this lysis buffer is also compatible with many other applications, including the Thermo Scientific Pierce BCA and 660 nm Protein Assays, protein purification and immunoassays (e.g., ELISA, Western blot).

Pierce IP Lysis Buffer is composed of 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol. The buffer does not contain protease or phosphatase inhibitors; however, if desired, inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail or Phosphatase Inhibitor Cocktail can be added just before use to prevent proteolysis and maintain phosphorylation of proteins.

Related Products
M-PER™ Mammalian Protein Extraction Reagent
RIPA Lysis and Extraction Buffer
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLiquid
Quantity100 mL
Volume (Metric)100 mL
Product LinePierce™
Product TypeIP Lysis Buffer
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3214638Certificate of AnalysisJun 06, 202587787
3216734Certificate of AnalysisMay 21, 202587788
3212486Certificate of AnalysisMay 05, 202587787
3212632Certificate of AnalysisApr 29, 202587788
AC411091Certificate of AnalysisApr 22, 202587787
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (7)

Citations & References
Abstract
A guide to selecting high-performing antibodies for Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) for use in Western Blot, immunoprecipitation and immunofluorescence.
Authors:Ayoubi R,Fotouhi M,Alende C,Southern K,Laflamme C,NeuroSGC/YCharOS/EDDU collaborative group,ABIF consortium
Journal:F1000Research
PubMed ID:39935523
Protein phosphatase 2A is a serine/threonine phosphatase with activity dependent on an associated regulatory subunit, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta (δ) isoform (PPP2R5D). PPP2R5D is the δ isoform in the B56 family of regulatory subunits. Abundantly expressed in the brain and involved in a broad range of ... More
The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence.
Authors:Ayoubi R,McDowell I,Fotouhi M,Southern K,McPherson PS,Laflamme C,NeuroSGC/YCharOS/EDDU collaborative group,ABIF Consortium
Journal:F1000Research
PubMed ID:37576538
Profilin-1, a member of the Profilin family, is a ubiquitously expressed protein that controls actin polymerization in a concentration-dependent manner. As mutations in the Profilin-1 gene have potential implications in neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to the scientific community. In this study, we characterized sixteen Profilin-1 ... More
Silencing LINC00665 inhibits cutaneous melanoma in vitro progression and induces apoptosis via the miR-339-3p/TUBB.
Authors:Liu Y,Ma S,Ma Q,Zhu H
Journal:Journal of clinical laboratory analysis
PubMed ID:35929185
BACKGROUND: LncRNAs are closely related to cutaneous melanoma (CM) tumorigenesis and metastasis, and it can affect the progression of CM by regulating cell proliferation, migration, invasion, apoptosis, and other cellular mechanisms. This study investigated the role of LINC00665 in CM. METHODS: Expressions of LINC00665, miR-339-3p, and tubulin beta chain (TUBB) ... More
A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
Authors:Ayoubi R,Southern K,Laflamme C,NeuroSGC/YCharOS Collaborative Group
Journal:F1000Research
PubMed ID:39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In ... More
Rac1 augments Wnt signaling by stimulating ß-catenin-lymphoid enhancer factor-1 complex assembly independent of ß-catenin nuclear import.
Authors:Jamieson C, Lui C, Brocardo MG, Martino-Echarri E, Henderson BR
Journal:
PubMed ID:26403202
ß-Catenin transduces the Wnt signaling pathway and its nuclear accumulation leads to gene transactivation and cancer. Rac1 GTPase is known to stimulate ß-catenin-dependent transcription of Wnt target genes and we confirmed this activity. Here we tested the recent hypothesis that Rac1 augments Wnt signaling by enhancing ß-catenin nuclear import; however, ... More
7 total citations

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