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Gibco™
RPMI 1640 Medium, no glutamine, no phenol red
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
| Catalog Number | Quantity |
|---|---|
| 32404014 | 500 mL |
Catalog number 32404014
Price (USD)
36.31
Each
-
Quantity:
500 mL
Price (USD)
36.31
Each
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
| Without |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality260 - 300 mOsm/kg
Product LineGibco™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesSodium Bicarbonate
Without AdditivesNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Media Formulations
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3144444Certificate of AnalysisMay 10, 202532404014
3090879Certificate of AnalysisMar 16, 202532404014
3023267Certificate of AnalysisFeb 19, 202532404014
2555063Certificate of AnalysisJan 29, 202532404014
2549528Certificate of AnalysisJan 29, 202532404014
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.