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Gibco™
MEM α, nucleosides, no phenol red
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negativeRead more
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| Catalog Number | Quantity |
|---|---|
| 41061029 | 500 mL |
| 41061037 | 10 x 500 mL |
Catalog number 41061029
Price (USD)
71.65
Online Exclusive
78.00Save 6.35 (8%)
Each
In stock
Quantity:
500 mL
Price (USD)
71.65
Online Exclusive
78.00Save 6.35 (8%)
Each
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
| With | Without |
| • Ribonucleosides | • Phenol Red |
| • Deoxyribonucleosides | |
| • L-glutamine |
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM α (Minimum Essential Medium α)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesLow Glucose, Glutamine, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without AdditivesNo HEPES, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3175879Certificate of AnalysisJun 13, 202541061029
3230562Certificate of AnalysisJun 06, 202541061029
3149790Certificate of AnalysisMay 11, 202541061029
3141657Certificate of AnalysisMay 07, 202541061029
3144492Certificate of AnalysisMar 28, 202541061029
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
The osmolality is listed in the COA for the particular lot number of the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.