Search Thermo Fisher Scientific
Certificates
SDS
Citations & References (2)
Gibco™
MEM, no glutamine, no phenol red
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be usedRead more
PromotionPromo code:RCELL25
Save up to 29% across the cell culture workflow - research begins with cells
From now until Dec 31, get up to 29% off media, sera, buffers, reagents and other cell culture products to advance your researchLearn More
| Catalog Number | Quantity |
|---|---|
| 51200038 | 500 mL |
Catalog number 51200038
Price (USD)
65.00
Each
Quantity:
500 mL
Price (USD)
65.00
Each
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| Without |
| • L-glutamine |
| • HEPES |
| • Phenol Red |
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesLow Glucose
Without AdditivesNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Frequently asked questions (FAQs)
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3245639Certificate of AnalysisJul 03, 202551200046, 51200038
3168562Certificate of AnalysisJun 27, 202551200046, 51200038
3224753Certificate of AnalysisMay 23, 202551200046, 51200038
3108086Certificate of AnalysisApr 12, 202551200046, 51200038
2549532Certificate of AnalysisJan 29, 202551200038
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSScientific Resources
Product Information
Citations & References (2)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Astrocytes in culture produce anandamide and other acylethanolamides.
Journal:J Biol Chem
PubMed ID:11916961
'Anandamide (arachidonylethanolamide) is an endocannabinoid that belongs to the acylethanolamide lipid family. It is produced by neurons in a calcium-dependent manner and acts through cannabinoid CB1 receptors. Other members of the acylethanolamide lipid family are also produced by neurons and act through G-protein-coupled receptors: homo-gamma-linolenylethanolamide (HEA) and docosatetraenylethanolamide (DEA) act
Contact and encirclement of glioma cells in vitro is an intrinsic behavior of a clonal human neural stem cell line.
Journal:PLoS One
PubMed ID:23240066
Pathotropic neural stem and/or progenitor cells (NSCs) can potentially deliver therapeutic agents to otherwise inaccessible cancers. In glioma, NSCs are found in close contact with tumor cells, raising the possibility that specificity of NSC contact with glioma targets originates in the tumor cells themselves. Alternatively, target preferences may originate, at
2 total citations