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Ion Xpress™ Barcode Adapters 1-16 Kit
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Ion Xpress™ Barcode Adapters 1-16 Kit
Ion Torrent™

Ion Xpress™ Barcode Adapters 1-16 Kit

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated forRead more
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Catalog NumberQuantity
44712501 kit
Catalog number 4471250
Price (USD)
3,075.00
Each
In stock
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Quantity:
1 kit
Recurring order eligible. Learn more »
Price (USD)
3,075.00
Each
Add to cart
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The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Features of the Ion Xpress™ Barcode Adapters 1-16 Kit include:

  • Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
  • Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
  • Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput

Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design

Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
CompatibilityGenomic DNA
For Use With (Application)Post-Light™ Ion Semiconductor Sequencing
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
LibrariesFragment Library
Product TypeBarcode Adapter
Quantity1 kit
Sequencing TypeGenome & DNA Sequencing
Shipping ConditionDry Ice
Throughput96 Samples per Run
Workflow StepLibrary Barcoding
Product LineIon Torrent
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Ion Xpress™ P1 Adapter, 1 tube, 320 μL, store at -10°C to -30°C
• Ion Xpress™ Barcode 1, 1 tube, 20 μL
• Ion Xpress™ Barcode 2, 1 tube, 20 μL
• Ion Xpress™ Barcode 3, 1 tube, 20 μL
• Ion Xpress™ Barcode 4, 1 tube, 20 μL
• Ion Xpress™ Barcode 5, 1 tube, 20 μL
• Ion Xpress™ Barcode 6, 1 tube, 20 μL
• Ion Xpress™ Barcode 7, 1 tube, 20 μL
• Ion Xpress™ Barcode 8, 1 tube, 20 μL
• Ion Xpress™ Barcode 9, 1 tube, 20 μL
• Ion Xpress™ Barcode 10, 1 tube, 20 μL
• Ion Xpress™ Barcode 11, 1 tube, 20 μL
• Ion Xpress™ Barcode 12, 1 tube, 20 μL
• Ion Xpress™ Barcode 13, 1 tube, 20 μL
• Ion Xpress™ Barcode 14, 1 tube, 20 μL
• Ion Xpress™ Barcode 15, 1 tube, 20 μL
• Ion Xpress™ Barcode 16, 1 tube, 20 μL
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Frequently asked questions (FAQs)

Do libraries constructed with the Ion Xpress Barcode adapters require balancing to help ensure equal representation on the Ion PGM or Proton instrument sequencing run?

No, the Ion Xpress Barcode adapters are designed with a 3 bp adapter sequence (GAT) at the 3' end of each barcode to help minimize adapter ligation bias. When pooling barcoded libraries, accurate library quantification is extremely important to help ensure even barcode representation on the sequencing run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many fragment or amplicon libraries can be made per barcode with the Ion Xpress Barcode Adapters kits?

The Ion Xpress Barcode Adapters kits are available as a set of 96 (Cat. No. 4474517) or in sets of 16 (Cat. Nos.4471250, 4474009, 4474518, 4474519, 4474520 or 4474521). Each barcode kit is sufficient for preparing ? 10 libraries per barcode (10 x 16 libraries or 10 x 96 libraries) for 100 ng input, or 2 libraries per barcode for 1 µg input.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I am planning to use the Oncomine BRCA Research Assay, Manual Library Preparation (Cat. No. A32840). Which other products would need I to order?

Here are the products you would need to order:

- Ion Xpress Barcode Adapters Kits
- Agencourt AMPure XP (Beckman Coulter Cat. No. A63880 or A63881)
- Ion Library Equalizer Kit (Cat. No. 4482298)

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