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Ion Xpress™ Barcode Adapters 1-96 Kit
The Ion Xpress™ Barcode Adapter 1-96 Kit provides an extensive set of 96 unique barcode adapters specifically designed and validatedRead more
| Catalog Number | Quantity |
|---|---|
| 4474517 | 1 kit |
Catalog number 4474517
Price (EUR)
12 930,00
Each
-
Quantity:
1 kit
Price (EUR)
12 930,00
Each
The Ion Xpress™ Barcode Adapter 1-96 Kit provides an extensive set of 96 unique barcode adapters specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, you may pool from 2 to 96 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the next generation sequencing workflow for a wide range of applications, including targeted resequencing.
Features of the Ion Xpress™ Barcode Adapter 1-96 Kit include:
- Enables multiplexing of 96 fragment library samples on a single sequencing chip with the use of robust molecular barcodes
- Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
- Robust error correction built in by design for added confidence in sample identification
Multiplexing with Barcode Adapters Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.
Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adapter sequencing required which results in more economical multiplexed sequencing runs.
Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
Sample TypeDNA
Sequencing TypeGenome and DNA Sequencing
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
For Use With (Application)Sequencing
LibrariesFragment Library
Product LineIon Torrent™
Product TypeBarcode Adapter Kit
Quantity1 kit
Workflow StepLibrary Barcoding
Unit SizeEach
Contents & Storage
6 boxes of 17 tubes, store at -20°C.
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Frequently asked questions (FAQs)
No, the Ion Xpress Barcode adapters are designed with a 3 bp adapter sequence (GAT) at the 3' end of each barcode to help minimize adapter ligation bias. When pooling barcoded libraries, accurate library quantification is extremely important to help ensure even barcode representation on the sequencing run.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The Ion Xpress Barcode Adapters kits are available as a set of 96 (Cat. No. 4474517) or in sets of 16 (Cat. Nos.4471250, 4474009, 4474518, 4474519, 4474520 or 4474521). Each barcode kit is sufficient for preparing ? 10 libraries per barcode (10 x 16 libraries or 10 x 96 libraries) for 100 ng input, or 2 libraries per barcode for 1 µg input.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
The TAG Sequencing Barcode Set contains adapters designed for Oncomine workflows using AmpliSeq HD technology and they can be used only with those library kits. This type of chemistry uses a molecular tag in order to track polymerase errors and increase the assay sensitivity. The Ion Xpress Barcode Adapters are not an alternative of the TAG Sequencing Barcode Set and vice versa. The list of the barcodes set compatible with a library kit is specified in the library manual.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.