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Dynabeads™ Streptavidin Trial Kit
The Dynabeads Streptavidin Trial Kit contains four different streptavidin-coated Dynabeads products making it more convenient to test out which ofRead more
| Catalog Number | Quantity |
|---|---|
| 65801D | 4 x 1 mL |
Catalog number 65801D
Price (USD)
338.65
Online Exclusive
378.00Save 39.35 (10%)
Each
-
Quantity:
4 x 1 mL
Price (USD)
338.65
Online Exclusive
378.00Save 39.35 (10%)
Each
The Dynabeads Streptavidin Trial Kit contains four different streptavidin-coated Dynabeads products making it more convenient to test out which of the beads perform best in a specific application. The kit contains 1 mL each of Dynabeads M-280 Streptavidin, Dynabeads M-270 Streptavidin, Dynabeads MyOne Streptavidin C1, and Dynabeads MyOne Streptavidin T1. The streptavidin-coated Dynabeads magnetic beads are monosized (1 or 2.8 μm in diameter depending on the product), monodispersed, and superparamagnetic. The products are intended for the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands.
Benefits of Dynabeads Streptavidin Trial Kit include:
• Performance—high sensitivity and reproducibility secure consistent results
• Efficiency—fast binding kinetics and high binding capacity
• Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System
Consistency and reproducibility
The monolayer of streptavidin leaves most of the biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer help ensure negligible leakage, giving batch consistency and reproducibility of results.
Applications
Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications such as immunoassays, purification of proteins, cells, extracellular vesicles/exosomes, viruses, exosomes, or phage display and bio-panning.
Learn more about our streptavidin products
Automation ready with the KingFisher Purification System
Dynabeads magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.
Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Streptavidin Trial Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bead TypeMagnetic Polystyrene
Certifications/ComplianceISO9001 and ISO13485
ColorBrown
Concentration10 mg/mL
DescriptionRecombinant streptavidin covalently coupled to Dynabeads
Diameter (Metric)1, 2.8 μm
For Use With (Application)Capture of Biotinylated Targets
For Use With (Equipment)KingFisher™ Sample Purification System, DynaMag™ magnets
High-throughput CompatibilityHigh-throughput Compatible
Ligand TypeStreptavidin
Product LineDynabeads™
Product TypeStreptavidin Magnetic Beads
Quantity4 x 1 mL
Regulatory StatusFor Research Use Only
Sample TypeAny Sample Type
Shelf Life36 months from date of manufacture
Shipping ConditionAmbient Temperature
Size DistributionCV <5%
SpeciesRecombinant
Surface FunctionalityTosylactivated-Hydrophobic (M-280 beads and MyOne T1), Carboxylic Acid-Hydrophilic (M-270 beads and MyOne C1)
TargetBiotin
FormatBeads in suspension
Isolation TechnologyMagnetic beads
Unit SizeEach
Contents & Storage
The Dynabeads Streptavidin Trial Kit contains 1 mL (10 mg/mL) each of four different streptavidin-coated Dynabeads so you can find the best match for your application.
• Dynabeads M-280 Streptavidin
• Dynabeads M-270 Streptavidin
• Dynabeads MyOne Streptavidin C1
• Dynabeads MyOne Streptavidin T1
Store at 2–8°C.
• Dynabeads M-280 Streptavidin
• Dynabeads M-270 Streptavidin
• Dynabeads MyOne Streptavidin C1
• Dynabeads MyOne Streptavidin T1
Store at 2–8°C.
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3261057Certificate of AnalysisJun 16, 202565801D
3252801Certificate of AnalysisJun 03, 202565801D
3240968Certificate of AnalysisMay 16, 202565801D
3220764Certificate of AnalysisApr 17, 202565801D
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
We do not recommend this as streptavidin becomes hydrophobic and aggregates during denaturation.
Which product to choose depends on the properties of your sample, the buffers and solutions applied, as well as the downstream application. In general, all Dynabeads Streptavidin beads can be used in applications involving biotinylated ligands; however, some beads may perform better than others in particular applications due to their characteristics. Dynabeads M-280 Streptavidin beads and Dynabeads MyOne Streptavidin T1 beads are commonly used for protein and nucleic acid applications. Dynabeads M-270 Streptavidin beads and MyOne Streptavidin C1 beads are preferred for nucleic acid diagnostics, specifically with samples that have a high concentration of chaotropic salts, immunoassays involving small biotinylated antigens, and in applications that are not compatible with BSA, as these beads are not blocked with BSA. Dynabeads MyOne Streptavidin beads offer increased binding capacity and slower sedimentation rate, making them ideal for automated applications and when larger amounts of a biotinylated compound or its specific target need to be isolated. Please see the selection guide here ( https://www.thermofisher.com/us/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads.html?icid=fr-strep-1).
You can assay the supernatant for unbound nucleic acid to determine the amount of nucleic acid bound to the Dynabeads Streptavidin beads. The concentration of nucleic acids can be checked by measuring the OD or by running them on a gel. Alternatively, the nucleic acids can be labeled radioactively and the concentration measured directly on the beads, or fluorescently and measured in the supernatant.
Our Dynabeads Streptavidin magnetic beads can be used directly in PCR or real-time PCR. However, you would have to empirically optimize the amount of beads to be used per volume of reaction.
The exact number of streptavidin molecules bound per bead is not measured, but is approximately 14-16 µg streptavidin per milligram Dynabeads M-280 Streptavidin magnetic beads.