Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Catalog Number | Quantity | Separation Range | Gel Percentage |
---|---|---|---|
A42135 | 10 Gels | 50 to 2000 bp | 2% |
A42100 | 10 Gels | 100 to 5000 bp | 1% |
A45202 | 2 x 10 Gels | 100 to 5000 bp | 1% |
A45203 | 5 x 10 Gels | 100 to 5000 bp | 1% |
A45204 | 2 x 10 Gels | 50 to 2000 bp | 2% |
A45205 | 5 x 10 Gels | 50 to 2000 bp | 2% |
A42136 | 10 Gels | 10 to 500 bp | 4% |
A45206 | 2 x 10 Gels | 10 to 500 bp | 4% |
Features of E-Gel agarose gels include:
Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide and UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.
Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel staining steps. Just load your samples and run.
Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, safe real-time monitoring of DNA migration is enabled without risk to the eyes or sample, as opposed to UV.
Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for southern blot analysis.
Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.
Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages.
SYBR Safe-stained gels can be visualized with a standard UV transilluminator, a laser-based scanner equipped with an excitation source in the UV range or between 470 and 530 nm, or a blue-light transilluminator.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Here are some suggestions:
- Try cleaning the cassettes with alcohol and Kimwipes wipers.
- Try cleaning the camera lens.
- Try to adjust the exposure time and brightness options of the documentation system you are using.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Please ensure that you have not overloaded the well and that the wells were not damaged during comb removal.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.
Find additional tips, troubleshooting help, and resources within ourNucleic Acid Gel Electrophoresis and Blotting Support Center.
Loading buffer is optional. Samples can be loaded directly into the wells if no buffer is used, or you can dilute them with deionized water or TE buffer. If you want to use a loading buffer, please see the recipes below:
E-Gel agarose gels (including EX)
10 mM Tris-HCl, pH 7.5
1 mM EDTA
0.005% bromophenol blue
0.005% xylene cyanol FF
E-Gel CloneWell II and E-Gel SizeSelect II agarose gels
10 mM Tris-HCl, pH 7.5
1 mM EDTA
Alternatively, you can use 10X BlueJuice Gel Loading Buffer or TrackIt Loading Buffer. Dilute this buffer 50- to 200-fold to obtain optimal results with E-Gel agarose gels.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Share catalog number, name or link