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Invitrogen™
RNAqueous™ Total RNA Isolation Kit
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
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| Catalog Number | Quantity | Product Type |
|---|---|---|
| AM1912 | 50 Preps | RNA Isolation Kit |
| AM10051G | 50 Catridges | Spin Columns |
Catalog number AM1912
Price (USD)
474.00
Each
-
Quantity:
50 Preps
Product Type:
RNA Isolation Kit
Price (USD)
474.00
Each
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
The RNAqueous kit provides:
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50–120 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product TypeRNA Isolation Kit
Quantity50 Preps
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells, Cells: ≤107, Tissue: ≤75 mg, Plant: ≤75 mg, Yeast: ≤108 cells, Virus: varies, Enzymatic Reactions: varies
Isolation TechnologySpin Column (Glass Fiber Filter)
Sample TypeBacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Unit SizeEach
Contents & Storage
• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
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Figures
Total RNA from several different tissues and species isolated using the RNAqueous™ Kit.
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3205506Certificate of AnalysisApr 07, 2025AM1912
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Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
RNAs less than 200 nt are lost using the columns in the RNAqeous family of kits. These kits are not suitable for isolation of 5S rRNA or tRNA. The RNAqueous-Micro Total RNA Isolation Kit does, however, offer a modified protocol to allow isolation of miRNA from the sample.
To prevent filter clogging with viscous samples or samples containing a large amount of tissue, perform a clarifying centrifugatoin at 10,000-15,000 x g and remove to a fresh tube prior to adding ethanol to the lysis/binding solution.
RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).
Citations & References (1)
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Citations & References
Abstract
An optimized real-time PCR to avoid species-/tissue-associated inhibition for H5N1 detection in ferret and monkey tissues.
Journal:ScientificWorldJournal
PubMed ID:22645461
The real-time PCR diagnostics for avian influenza virus H5N1 in tissue specimens are often suboptimal, since naturally occurring PCR inhibitors present in samples, or unanticipated match of primer to unsequenced species' genome. With the principal aim of optimizing the SYBR Green real-time PCR method for detecting H5N1 in ferret and
1 total citations