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Citations & References (71)
Invitrogen™
DiD' solid; DiIC18(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)
The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is supplied as a solid form to facilitateRead more
| Catalog Number | Quantity |
|---|---|
| D7757 | 10 mg |
Catalog number D7757
Price (USD)
234.65
Online Exclusive
242.00Save 7.35 (3%)
Each
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Quantity:
10 mg
Price (USD)
234.65
Online Exclusive
242.00Save 7.35 (3%)
Each
The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is supplied as a solid form to facilitate direct application of crystals to membranes and is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (∼1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Quantity10 mg
Shipping ConditionRoom Temperature
Product TypeLiphophilic Tracer
SubCellular LocalizationCell Membranes, Lipids
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
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Figures
Neurons in ventrobasal nucleus of newborn mouse thalamus. DiI, DiD.
Chemical Structure
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3148253Certificate of AnalysisMar 30, 2025D7757
3043930Certificate of AnalysisNov 25, 2024D7757
2720330Certificate of AnalysisOct 16, 2023D7757
2570392Certificate of AnalysisFeb 23, 2023D7757
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Safety Data Sheets
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Molecular Probes® Handbook
Frequently asked questions (FAQs)
This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
A typical method is to label one cell line with orange fluorescent DiI C18 and the other cell line with green fluorescent DiO C18. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (71)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.
Journal:Nat Methods
PubMed ID:20581828
Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We
Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.
Journal:Mol Biol Cell
PubMed ID:10198064
'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
Journal:J Cell Biol
PubMed ID:8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16263923
'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with
In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.
Journal:J Immunol
PubMed ID:9144465
'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate
71 total citations