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Invitrogen™
LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit is used to determine the viability of cells prior to the fixationRead more
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| Catalog Number | Quantity |
|---|---|
| L34959 | 200 Assays |
| L34967 also known as L-34967 | 80 assays |
| L34968 | 400 Assays |
Catalog number L34959
Price (USD)
391.00
Each
In stock
Quantity:
200 Assays
Price (USD)
391.00
Each
The LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer.
• Stable—dyes are freeze dried in separate vials to maintain stability
• Robust—staining pattern is the same before and after fixation
• Low compensation—minimal spectral overlap between other fluorophores
View a selection guide for all fixable viability dyes for flow cytometry.
Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Yellow Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.
Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.
Low compensation
The LIVE/DEAD™ Fixable Yellow Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser. The yellow-fluorescent reactive dye has an excitation maximum of ∼405 nm, but it is excited well with the 405 nm violet laser, and it has an emission maxima of ∼570 nm, so it can be collected in the second or third channel on most violet laser flow cytometers.
How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.
Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.
• Stable—dyes are freeze dried in separate vials to maintain stability
• Robust—staining pattern is the same before and after fixation
• Low compensation—minimal spectral overlap between other fluorophores
View a selection guide for all fixable viability dyes for flow cytometry.
Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Yellow Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.
Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.
Low compensation
The LIVE/DEAD™ Fixable Yellow Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser. The yellow-fluorescent reactive dye has an excitation maximum of ∼405 nm, but it is excited well with the 405 nm violet laser, and it has an emission maxima of ∼570 nm, so it can be collected in the second or third channel on most violet laser flow cytometers.
How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.
Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityImpermeant
Cell TypeEukaryotic Cells
DescriptionLIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation
Detection MethodFluorescence
Dye TypeLIVE/DEAD™ Fixable Yellow Dead Cell Stain
FormSolid
FormatTube(s)
Quantity200 Assays
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
ColorYellow
Emission570
Excitation Wavelength Range405 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product LineLIVE/DEAD
Product TypeStain
Unit SizeEach
Contents & Storage
Contains 5 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.
Store at -20°C.
Store at -20°C.
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How It Works: LIVE/DEAD® Fixable Dead Cell Stain Kit
Live and dead cells distinguished by flow cytometry
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Lot #Certificate TypeDateCatalog Number(s)
3148322Certificate of AnalysisJul 21, 2025L34968
2980658Certificate of AnalysisJul 29, 2024L34967
2866022Certificate of AnalysisMar 15, 2024L34968
2775959Certificate of AnalysisDec 05, 2023L34967
2729837Certificate of AnalysisOct 30, 2023L34959
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Citations & References (19)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.
Journal:PLoS One
PubMed ID:22720031
'Cytokine expression patterns of T cells can be regulated by pre-commitment to stable effector phenotypes, further modification of moderately stable phenotypes, and quantitative changes in cytokine production in response to acute signals. We showed previously that the epidermal growth factor family member Amphiregulin is expressed by T cell receptor-activated mouse
IL-2-dependent adaptive control of NK cell homeostasis.
Journal:J Exp Med
PubMed ID:23650439
'Activation and expansion of T and B lymphocytes and myeloid cells are controlled by Foxp3(+) regulatory T cells (T reg cells), and their deficiency results in a fatal lympho- and myeloproliferative syndrome. A role for T reg cells in the homeostasis of innate lymphocyte lineages remained unknown. Here, we report
Host immunity and pathogen strain contribute to intestinal disaccharidase impairment following gut infection.
Journal:J Immunol
PubMed ID:21873528
'Infection or other inflammatory insults in the small intestine often result in reduced disaccharidase enzyme levels. Using a mouse model of giardiasis, we examined the role of host immunity and pathogen virulence in mediating disaccharidase deficiency postinfection (p.i.). C57BL/6J mice were infected with two strains, WB and GS, of the
Functional recovery after peripheral nerve injury is dependent on the pro-inflammatory cytokines IL-1ß and TNF: implications for neuropathic pain.
Journal:J Neurosci
PubMed ID:21880915
'IL-1ß and TNF are potential targets in the management of neuropathic pain after injury. However, the importance of the IL-1 and TNF systems for peripheral nerve regeneration and the mechanisms by which these cytokines mediate effects are to be fully elucidated. Here, we demonstrate that mRNA and protein levels of
Tadalafil reduces myeloid-derived suppressor cells and regulatory T cells and promotes tumor immunity in patients with head and neck squamous cell carcinoma.
Journal:
PubMed ID:25320361
Myeloid-derived suppressor cells (MDSC) and regulatory T cells (Treg) play a key role in the progression of head and neck squamous cell carcinoma (HNSCC). On the basis of our preclinical data demonstrating that phosphodiesterase-5 (PDE5) inhibition can modulate these cell populations, we evaluated whether the PDE5 inhibitor tadalafil can revert
19 total citations