Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Find Nucleic Acid Purification and Analysis technical tips, troubleshooting help, and resources within our new Nucleic Acid Purification and Analysis Support Center.
For accurate quantification and a streamlined workflow try our new Quant-iT PicoGreen ReadyPlates!
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Invitrogen™

Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
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Catalog NumberQuantityProduct TypePackaging Type
P7589Promo Image1 mL kitdsDNA Assay Kit1 x 1 mL Bottle
P11496Promo Image10 x 100 μL kitdsDNA Assay Kit10 x 100 μL Tubes
P7581Promo Image1 mLdsDNA Reagent1 x 1 mL Bottle
P11495Promo Image10 x 100 μLdsDNA Reagent10 x 100 μL Tubes
Catalog number P7589
Price (USD)
667.65
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700.00
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Quantity:
1 mL kit
Product Type:
dsDNA Assay Kit
Packaging Type:
1 x 1 mL Bottle
Recurring order eligible. Learn more »
Price (USD)
667.65
Online Exclusive
700.00
Save 32.35 (5%)
Each
Add to cart
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Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers.
Rapidly detect as little as 0.25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA

Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200 Assays (2 mL assay volume)
Packaging Type1 x 1 mL Bottle
Product LinePICOGREEN, Quant-iT
Product TypedsDNA Assay Kit
Quantitation Range50 pg to 2 μg
Quantity1 mL kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach
Have questions about this product? Ask our AI assisted search.
How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?
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Can I make my own assay for the Qubit Fluorometer?
Can the Qubit kits give an indication of sample quality in nucleic acid samples?
What is the difference between the Quant-iT PicoGreen DNA, Quant-iT DNA, and Qubit DNA assays?
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Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Figures

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3223921Certificate of AnalysisJun 18, 2025P7589
3148355Certificate of AnalysisJun 01, 2025P11496
3148355Certificate of AnalysisJun 01, 2025P11496
3148276Certificate of AnalysisMay 18, 2025P7589
3144317Certificate of AnalysisMay 14, 2025P7589
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Citations & References (355)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:16517648
Mapping of multidrug resistance gene 1 and multidrug resistance-associated protein isoform 1 to 5 mRNA expression along the human intestinal tract.
Authors:Zimmermann C,Gutmann H,Hruz P,Gutzwiller JP,Beglinger C,Drewe J
Journal:Drug metabolism and disposition: the biological fate of chemicals
PubMed ID:15523049
Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling.
Authors:Hopwood A, Oldroyd N, Fellows S, Ward R, Owen SA, Sullivan K
Journal:Biotechniques
PubMed ID:9232218
Exo-proofreading, a versatile SNP scoring technology.
Authors:Cahill P, Bakis M, Hurley J, Kamath V, Nielsen W, Weymouth D, Dupuis J, Doucette-Stamm L, Smith DR
Journal:Genome Res
PubMed ID:12695330
We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3'-to-5' exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3' nucleotide base, and can be implemented in a variety of formats including ... More
Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses.
Authors:Weinbauer MG, Fritz I, Wenderoth DF, Höfle MG
Journal:Appl Environ Microbiol
PubMed ID:11872453
The aim of this study was to develop a protocol for the simultaneous extraction from bacterioplankton of RNA and DNA suitable for quantitative molecular analysis. By using a combined mechanical and chemical extraction method, the highest RNA and DNA yield was obtained with sodium lauryl sarcosinate-phenol or DivoLab-phenol as the ... More
355 total citations

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