ADN polimerasa Taq, recombinante
ADN polimerasa <i>Taq</i>, recombinante
Invitrogen™

ADN polimerasa Taq, recombinante

La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de plantillas monocatenarias en presencia deMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoN.º de reacciones
10342053100 reacciones
10342020500 reacciones
103420461500 reacciones
103421785000 reacciones
Número de catálogo 10342053
Precio (USD)
-
N.º de reacciones:
100 reacciones
Pedido a granel o personalizado
Ask our AI about this Product
La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de plantillas monocatenarias en presencia de dNTP y un primer. La enzima se compone de un polipéptido único con un peso molecular de 94 kDa. Tiene una actividad de ADN polimerasa de 5´→3´ y una actividad de exonucleasa de 5´→3´.

Con nuestra ADN polimerasa Taq podrá:

• Posibilidad de seleccionar la enzima nativa o recombinante
• Amplificación de productos de PCR con un tamaño máximo de 5 kb
• Una enzima que posee licencia y está certificada para PCR

Aplicaciones
La ADN polimerasaTaq es adecuada para su uso en la amplificación de ADN a partir de complejos moldes genómicos, virales y plasmídicos, RT-PCR, ADNss de secuenciación y secuenciación de ciclos.

Fuente
La enzima recombinante se purifica mediante el gen de ADN polimerasa Thermus aquaticus clonado expresado en E. coli.

Definición de unidad
Una unidad de ADN polimerasa Taq es la cantidad de enzima necesaria para incorporar 10 nmoles de desoxirribonucleótido en ADN en 30 min a 74 °C.

Para un rendimiento superior de PCR, recomendamos ADN polimerasa DreamTaq.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración5 U/µl
Actividad exonucleasa5' - 3'
Fidelidad (frente a Taq)1 X
FormatoEnzima independiente
Inicio en calienteNo
N.º de reacciones100 reacciones
Sobrante3'-A
PolimerasaADN polimerasa Taq
Tipo de productoADN polimerasa
Cantidad100 unidades
Formato de reacciónComponentes separados
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tamaño (producto final)5 kb o menos
Material de partidaADN
Para utilizar con (aplicación)Standard PCR
GC-Rich PCR PerformanceBajo
Método de PCRqPCR, PCR estándar
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Contiene:
• 20 μl de ADN polimerasa Taq (5 U/μl)
• Tampón para PCR 10X de 1,25 ml (200 mm de Tris-HCl, pH de 8,4 y 500 mm de KCl)
• 1 ml de cloruro magnésico (50 mm)

Almacenar a una temperatura de entre -30 °C y -10 °C en un congelador que no forme escarcha. Estabilidad garantizada durante seis meses si se almacena correctamente.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

Customers who viewed this item also viewed



Documentos y descargas

Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3144934Certificate of Analysis30 jun 202510342053
3171747Certificate of Analysis20 jun 202510342178
3130582Certificate of Analysis24 mar 202510342053
3074097Certificate of Analysis27 feb 202510342178
3103953Certificate of Analysis06 ene 202510342053
Se muestran 5 resultados, busque arriba un certificado específico

Hojas de datos de seguridad

Citations & References (11)

Citations & References
Abstract
Both DNA and histone fold sequences contribute to archaeal nucleosome stability.
Authors: Bailey Kathryn A; Marc Frederic; Sandman Kathleen; Reeve John N;
Journal:J Biol Chem
PubMed ID:11751933
'The roles and interdependence of DNA sequence and archaeal histone fold structure in determining archaeal nucleosome stability and positioning have been determined and quantitated. The presence of four tandem copies of TTTAAAGCCG in the polylinker region of pLITMUS28 resulted in a DNA molecule with increased affinity (DeltaDeltaG of approximately 700 ... More
CCAAT/enhancer-binding proteins beta and delta mediate the repression of gene transcription of cartilage-derived retinoic acid-sensitive protein induced by interleukin-1 beta.
Authors: Okazaki Ken; Li Jian; Yu Hua; Fukui Naoshi; Sandell Linda J;
Journal:J Biol Chem
PubMed ID:12072435
'Cartilage-derived retinoic acid-sensitive protein (CD-RAP) is a secreted protein expressed by chondrocytes; the expression is repressed by interleukin 1 beta (IL-1 beta). To investigate the transcriptional mechanism, by which CD-RAP expression is suppressed by IL-1 beta, deletion constructs of the mouse CD-RAP promoter were transfected into rat chondrocytes treated with ... More
alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer.
Authors: Rubin Mark A; Zhou Ming; Dhanasekaran Saravana M; Varambally Sooryanarayana; Barrette Terrence R; Sanda Martin G; Pienta Kenneth J; Ghosh Debashis; Chinnaiyan Arul M;
Journal:JAMA
PubMed ID:11926890
'Edited due to space constraints: CONTEXT: Molecular profiling of prostate cancer has led to the identification of candidate biomarkers and regulatory genes. Discoveries from these genome-scale approaches may have applicability in the analysis of diagnostic prostate specimens. OBJECTIVES: To determine the expression and clinical utility of alpha-methylacyl coenzyme A racemase ... More
The Salmonella enterica serovar typhimurium-encoded type III secretion systems can translocate Chlamydia trachomatis proteins into the cytosol of host cells.
Authors:Ho TD, Starnbach MN,
Journal:Infect Immun
PubMed ID:15664932
Chlamydia trachomatis is an obligate, intracellular pathogen that is a major cause of preventable blindness and infertility worldwide. Although the published genome sequence suggests that C. trachomatis encodes a type III secretion system, the lack of genetic tools for studying Chlamydia has hindered the examination of this potentially important class ... More
Novel isoforms of the sodium channels Nav1.8 and Nav1.5 are produced by a conserved mechanism in mouse and rat.
Authors:Kerr NC, Holmes FE, Wynick D,
Journal:J Biol Chem
PubMed ID:15047701
The voltage-gated sodium channel Nav1.8 is only expressed in subsets of neurons in dorsal root ganglia (DRG), trigeminal and nodose ganglia. We have isolated mouse partial-length Nav1.8 cDNA clones spanning exon 17 sequence which have 17 nucleotide substitutions and 12 predicted amino acid differences from the published sequence. The absence ... More
11 total citations

Compartir número de catálogo, nombre o enlace.

1x1 image pixel for data collection