RIPA Lysis and Extraction Buffer
RIPA Lysis and Extraction Buffer
Thermo Scientific™

RIPA Lysis and Extraction Buffer

Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysisRead more
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Catalog NumberQuantity
89900100 mL
89901250 mL
Catalog number 89900
Price (CNY)
641.00
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Ends: 31-Dec-2025
867.00
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100 mL
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Price (CNY)
641.00
Online Exclusive
Ends: 31-Dec-2025
867.00
Save 226.00 (26%)
Each
Add to cart
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Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

Features of RIPA Buffer:

Convenient—ready-to-use solution; no need to assemble and prepared components yourself
Flexible—compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
Versatile—enables extraction of cytoplasmic, membrane and nuclear proteins
Disclosed formulation—contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues

This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.

RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.

Related Products
Pierce™ IP Lysis Buffer
M-PER™ Mammalian Protein Extraction Reagent
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLiquid
Quantity100 mL
Volume (Metric)100 mL
Product TypeExtraction Buffer
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.
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Frequently asked questions (FAQs)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?

RIPA buffer produces a particularly strong absorbance signal at the 280 nm wavelength. As a result, it will either over estimate or under estimate protein concentrations and interfere with the protein purity ratio.
Protein samples in RIPA buffer should be quantified via the Pierce Protein 660 or BCA colorimetric assays using a full spectrum NanoDrop model.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?

Low phosphorylation is usually due to phosphatase activity. We recommend adding a Halt Phosphatase Inhibitor Cocktail to the buffer before use.
Alternatively, the protein is not phosphorylated or phosphorylated at a low level.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?

Proteolysis indicates that no protease inhibitors were added. We recommend adding a Halt Protease Inhibitor Cocktail to the RIPA Lysis and Extraction Buffer before use.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

I am getting a low concentration of proteins when using the RIPA Lysis and Extraction Buffer. What could be a possible cause and solution?

Most likely excess buffer was used. Use less buffer (e.g., 0.25 mL to 0.5 mL per 75cm^2 flask containing 5 x 10^6 cells), but use a sufficient amount to cover the entire plate.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

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3214589Certificate of AnalysisJul 02, 202589901
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Citations & References (10)

Citations & References
Abstract
Not just fat: investigating the proteome of cetacean blubber tissue.
Authors:Kershaw JL,Botting CH,Brownlow A,Hall AJ
Journal:Conservation physiology
PubMed ID:29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This ... More
Comparison of methods to isolate proteins from extracellular vesicles for mass spectrometry-based proteomic analyses.
Authors:Subedi P,Schneider M,Philipp J,Azimzadeh O,Metzger F,Moertl S,Atkinson MJ,Tapio S
Journal:Analytical biochemistry
PubMed ID:31401005
Extracellular vesicles (EVs) are cell-derived membrane-bound organelles that have generated interest as they reflect the physiological condition of their source. Mass spectrometric (MS) analyses of protein cargo of EVs may lead to the discovery of biomarkers for diseases. However, for a comprehensive MS-based proteomics analysis, an optimal lysis of the ... More
Alternative direct-to-amplification cell lysis techniques for forensically relevant non-sperm cells.
Authors:Arya R,Hudson BC,Green TD
Journal:Journal of forensic sciences
PubMed ID:37779342
While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Recently, an ... More
Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
Authors:O'Leary MF,Jackman SR,Bowtell JL
Journal:European journal of nutrition
PubMed ID:38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples ... More
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function.
Authors:Hosseinkhani B,Duran G,Hoeks C,Hermans D,Schepers M,Baeten P,Poelmans J,Coenen B,Bekar K,Pintelon I,Timmermans JP,Vanmierlo T,Michiels L,Hellings N,Broux B
Journal:Fluids and barriers of the CNS
PubMed ID:38114994
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes ... More
10 total citations

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