BenchStable™ RPMI 1640
BenchStable™ RPMI 1640
Gibco™

BenchStable™ RPMI 1640

Green features
Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspensionRead more
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Catalog NumberQuantity
A4192301500 mL
A419230210 x 500 mL
Catalog number A4192301
Price (CNY)
400.60
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Ends: 31-Dec-2025
508.00
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Quantity:
500 mL
Price (CNY)
400.60
Online Exclusive
Ends: 31-Dec-2025
508.00
Save 107.40 (21%)
Each
Add to cart
Ask our AI about this Product

Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable RPMI 1640 Medium has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional RPMI 1640 Medium when supplemented with ≥10% fetal bovine serum (FBS).

This RPMI 1640 Medium is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPES

Using BenchStable RPMI 1640 Medium
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L) and so requires a 5–10% CO2 environment to maintain physiological pH. RPMI 1640 Medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable RPMI 1640 Medium contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Concentration1 X
Green FeaturesEnergy efficient, Fewer resources used, Sustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 9001: 2015 standard
Product LineBenchStable™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesGlutaMAX, Phenol Red, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Store at room temperature. Protect from light.
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Frequently asked questions (FAQs)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3047723Certificate of AnalysisJun 23, 2025A4192301
3002243Certificate of AnalysisOct 17, 2024A4192301
2996507Certificate of AnalysisSep 09, 2024A4192301
2955131Certificate of AnalysisJul 09, 2024A4192301
2882317Certificate of AnalysisJun 12, 2024A4192301
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Citations & References (2)

Citations & References
Abstract
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
FELICX: A robust nucleic acid detection method using flap endonuclease and CRISPR-Cas12.
Authors:Chang MW
Journal:Biosensors & bioelectronics
PubMed ID:36527830
2 total citations

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