Neon™ Transfektionssystem 100 μl-Kit

The Neon Transfection instrument (Cat. No. MPK5000 and MPK5000S) has been discontinued. The Neon consumables (Cat. No. MPK1025, MPK10025, MPK1096, MPK10096, and MPT100) will be discontinued on September 30, 2025 or while supplies last. Learn about the benefits of the new Neon NxT Electroporation System with improved usability, more protocols with plate setup, and connectivity.

Zertifikate

SDB

Invitrogen™

Neon™ Transfektionssystem 100 μl-Kit

Das Neon™ Transfection System 100 µl-Kit ist speziell für die Verwendung mit dem Neon™ Transfection System konzipiert. Sie können diesesWeitere Informationen

Ansicht ändern

Katalognummer	Anzahl Reaktionen
MPK10096	96 x 2
MPK10025	50 Reaktionen

2 Optionen

Katalognummer MPK10096

Preis (EUR)

2.180,00

Each

Zum Warenkorb hinzufügen

Anzahl Reaktionen:

96 x 2

Preis (EUR)

2.180,00

Each

Zum Warenkorb hinzufügen

Das Neon™ Transfection System 100 µl-Kit ist speziell für die Verwendung mit dem Neon™ Transfection System konzipiert. Sie können dieses Kit für Transfektionsvolumen von 100 µl verwenden, die 5 × 10⁵ bis 2 × 10⁶ adhärente Zellen oder 1 × 10⁶ bis 5 × 10⁶ Suspensionszellen enthalten. Zellen, die mit den mitgelieferten 100 µl Neon™ Spitzen transfiziert wurden, sind dann bereit, gewaschen und in eine 6-Well-, 60 mm-, oder 10 cm-Petrischale überführt zu werden, je nach Ihrem Protokoll.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

KlassifikationOhne Stoffe tierischen Ursprungs, chemisch definiert, proteinarm, Xeno-frei, reduzierte Wachstumsfaktoren, reduziertes Serum

Konzentration5 x 10^5 bis 2 x 10^6 Zellen/ml (adhärente Zellen), 1 x 10^6 bis 5 x 10^6 Zellen/ml

Zur Verwendung mit (Anwendung)Transfektion

Zur Verwendung mit (Geräte)Neon™ Transfection System konzipiert.

Anzahl Reaktionen96 x 2

ProduktlinieNeon™

ProdukttypTransfektionssystemkit

Menge96 x 2 Reaktionen

Serum-kompatibelJa

VersandbedingungRaumtemperatur

Ausgangsmaterialmenge2 x 10^4 – 6 x 10^8 Zellen; variiert je nach Zelltyp

SystemtypNeon™ Transfection System konzipiert.

Probenvolumen (metrisch)100 µl

ZelltypStammzelle, Primärzelle, schwer transfizierbare Zelle

Format6-Well-Platte, 12-Well-Platte, 24-Well-Platte, 48-Well-Platte, 96-Well-Platte, Kolben

ProbentypPlasmid-DNA, Synthetische siRNA

Transfection TechniqueElektroporation

Unit SizeEach

Inhalt und Lagerung

• 30 ml Resuspensionspuffer R
• 30 ml Resuspensionspuffer T
• 2 x 150 ml Elektrolytpuffer E2
• 96 x 100 µl Neon™ Spitzen
• 20 Neon™ Elektroporationsröhrchen

Die Puffer-Box bei 4 °C und die Röhrchen-Box bei Raumtemperatur lagern.

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Häufig gestellte Fragen (FAQ)

What are the common causes for low cell survival rate using the Neon device?

Here are possible causes for low transfection efficiency using the Neon device:

1. Sub-optimal electrical parameters
2.Poor plasmid quality such as endotoxin contamination
3 .Plasmid preparation containing high salt
4. Plasmid quantity too high
5. Cells are stressed or damaged
6. Using same Neon tip more than two times
7. Microbubbles in tip, causing arcing

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What are the common causes for low transfection efficiency using the Neon device?

Here are possible causes for low transfection efficiency using the Neon device:

1. Sub-optimal electrical parameters
2. Plasmid preparation containing high salt
3. Plasmid larger than 10 kb
4. Plasmid concentration too low
5. Cells are stressed, damaged, or contaminated by Mycoplasma
6.Cell density too low or too high
7. Cells with high passage number

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How should I determine siRNA transfection efficiency with the Neon Transfection System?

To determine the Neon transfection efficiency for siRNA, we recommend transfecting the cells with a fluorescent-labeled negative control siRNA (BLOCK-iT Fluorescent Oligo, Cat. No. 13750062) and measuring the transfection efficiency by the percentage of fluorescent-stained cells among viable cells. However, keep in mind that there is a caveat with this approach: the transfection efficiency determined by fluorescent-labeled negative control siRNA may over-estimate the transfection efficiency, as fluorescence detection with a microscope does not distinguish the siRNA that enters the cell from the siRNA that sticks to the cell membrane. To measure transfection efficiency more accurately, one needs to transfect the cells with a positive control siRNA such as the one that targets a house-keeping gene, and measure the knockdown of target RNA or protein.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How should I determine the cell viability and transfection efficiency with the Neon Transfection System?

Cell viability is the number of cells that are confirmed viable from a total cell population. Transfection efficiency is the number of cells that are successfully expressing your construct out of the total number of viable cells (i.e., GFP-positive cells).

Cell viability can be determined by staining cells with propidium iodide or by the trypan blue exclusion method. For adherent cells, cell detachment can be performed using Trypsin or TrypLE Express enzyme prior to staining. Transfection efficiency can be determined using a fluorescence microscope with filter settings appropriate for the detection of GFP (emission: 509 nm). Cells may be counted either by FACS or using the Countess Automated Cell Counter.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

After Neon electroporation, what is the best time point for analysis of siRNA knockdown?

As the stability and half-life of various mRNAs and their protein products varies, it is important to empirically determine the best time points for assessing target knockdown. For example, it has been documented that in mammalian cells, mRNA half-life can range from minutes to days (Ross J, 1995, Microbiol Rev 59:423–450) while the half-life of protein products can range from less than a few minutes to several days. In general, the recommended time course ranges from 12 to 72 hours to knock down target mRNA and 24 to 96 hours to adequately knock down target proteins. We recommend measuring mRNA knockdown by qPCR at 8, 24, 48, 72, and 96 hours post-electroporation to determine the time point for maximum knockdown. Also, perform time-course analysis to determine protein knockdown by ELISA (more accurate) or immunoblotting (less accurate).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all Neon™ Transfektionssystem 100 μl-Kit FAQs