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Applied Biosystems™
Fast SYBR™ Green Master Mix
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution forRead more
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| Catalog Number | Quantity |
|---|---|
| 4385612 | 1 x 5 mL |
| 4385610 | 1 x 1 mL |
| 4385616 | 2 x 5 mL |
| 4385617 | 5 x 5 mL |
| 4385618 | 10 x 5 mL |
| 4385614 | 1 x 50 mL |
Catalog number 4385612
Price (EUR)
445,65
Online Exclusive
642,00Save 196,35 (31%)
Each
In stock
Quantity:
1 x 5 mL
Price (EUR)
445,65
Online Exclusive
642,00Save 196,35 (31%)
Each
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision
Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision
Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
FormatVial
No. of Reactions500 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product LineSYBR™
Product TypeReal Time PCR SYBR Master Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity1 x 5 mL
Sample TypeDNA (Genomic), cDNA
Shipping ConditionDry Ice
Sufficient For500 Reactions of 20 μL Each
Volume5 mL
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
Label or DyeSYBR Green
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
Contains 1 × 5 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 500 reactions of 20 μL each.
Store at -5 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Store at -5 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
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Lot #Certificate TypeDateCatalog Number(s)
3258685Certificate of AnalysisJun 16, 20254385612
3227181Certificate of AnalysisJun 05, 20254385610
3221656Certificate of AnalysisJun 04, 20254385614
3236510Certificate of AnalysisMay 27, 20254385612
3243916Certificate of AnalysisMay 22, 20254385612
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Safety Data Sheets
SDSProduct Information
Scientific Resources
Frequently asked questions (FAQs)
Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.
DNA synthesis rate of the AmpliTaq Gold Fast DNA Polymerase LD is 1 min/kb.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
Citations & References (1)
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Citations & References
Abstract
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation
1 total citations